Xenbase Image ID: 133106

Figure 5 Expression and localization of Xzar2 RNA. (A) RT-PCR analysis of Xzar2 expression during early development. The ‘–RT’ lane contained all reagents except for reverse transcriptase and was used as a negative control. Ornithine decarboxylase (ODC) was used as a loading control. (B) Whole-mount in situ hybridization using anti-sense Xzar2 probe. At blastula and gastrula stages, Xzar2 was expressed in the ectodermal and the mesodermal regions. (a) Lateral view of a blastula embryo (stage 8). (b) Lateral view of an early gastrula embryo (stage 10). (c) Vegetal view of a late gastrula embryo (stage 12), as even shown in the corresponding vertical section (c′). (C) Intracellular localization of Xzar2. HEK-293 cells were transfected with EGFPN1- Xzar2 construct. Images were obtained using a confocal microscope. (a) Phase contrast. (b) Distribution of Xzar2 in a nucleus. (c) Merge image of (a) and (b). Bar = 100 μm. Image published in: Nakajima Y et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue zar1l.S xzar2, Z3CXXC7, zar-2, zar2 X. laevis Throughout NF stage 8 to NF stage 10 animal cap animal hemisphere animal zar1l.S xzar2, Z3CXXC7, zar-2, zar2 X. laevis Sometime during NF stage 8 to NF stage 12 animal cap ectoderm mesoderm involuting marginal zone circumblastoporal collar involuted dorsal mesoderm involuted ventral mesoderm Image source: Published Larger Image Printer Friendly View

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