XB-IMG-135240
Xenbase Image ID: 135240
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Figure 2. Expression of Nrarp mirrors that of other Notch pathway genes. Xenopus embryos were stained for the expression of Nrarp RNA using whole-mount in situ hybridization. (A) Nrarp expression at early neurulae stages. Shown is a dorsal view with anterior oriented to the left. Note that Nrarp RNA is expressed at high levels in the neural plate, localizing to the three bilateral domains where the Notch pathway is known to operate during the formation of the primary neurons (M, medial; I, intermediate; L, lateral). In addition, NrarpRNA can be detected in the paraxial mesoderm (PM). (B,C) For comparison, note that the expression pattern of Nrarp is similar to that of XDelta1(B) and ESR1 (C). (D) Expression ofNrarp RNA in a tadpole embryo stained in whole mount. Shown is a side view of the anterior end with anterior oriented to the left. Note expression in the eye (EY), neural tube (NT), and branchial arches (BA). (E) Tissue section of an embryo as in panel Dshows that the expression of Nrarp RNA in the neural tube is confined to the ventricular zone where neurogenesis occurs. (F) Expression of Nrarp occurs at high levels in the outgrowing tailbud where Notch signaling is known to play a role in segmentation. High levels of Nrarp expression are apparent in the most posterior portion of the presomitic mesoderm called the tailbud domain (TBD) and in segments in a region containing prospective somites. This expression pattern in the presomitic mesoderm closely parallels that of XDelta2 and two Notch target genes,ESR4 and ESR5. Image published in: Lamar E et al. (2001) Copyright © 2001. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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