Xenbase Image ID: 1354

Figure 2. In situ hybridization analysis of developmental expression of the Xenopus alpha -MHC and vMHC genes. A: Lateral view of stage 26 embryos showing alpha -MHC transcripts in the bisymmetric heart patches. B: Ventral view of embryo in A. C: Transverse section through the heart patch of stage 26 embryo. D: Lateral view of stage 30 embryo showing increased alpha -MHC expression in precardiac tissues (arrow). E: Lateral view of stage 30 embryo showing an absence of vMHC expression at this stage of development. F: Lateral view of stage 38 embryo showing alpha -MHC expression in the heart, muscles of the jaw (jm), and the lymph heart (lh). G: Transverse section through stage 36 heart shows that alpha -MHC expression is confined to the myocardium. H: Transverse section through the lymph heart (stage 38) stained for alpha -MHC. I,J: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart showing that alpha -MHC is expressed in all chambers and outflow tract. K,L: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart, showing restricted expression of vMHC in a ring around the outflow tract and the atria at the junction with the ventricle. M: Section in situ of adult Xenopus heart shows that alpha -MHC is expressed throughout the myocardium, including the atria (a) and outflow tract. N: Section in situ hybridization of adult Xenopus heart shows that vMHC is expressed in the myocardium of the outflow tract and ventricle but not the atria. Image published in: Garriock RJ et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue myh15.L myh7, vMHC X. laevis Throughout adult frog stage cardiac ventricle outflow tract heart myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout adult frog stage myocardium left atrium right atrium outflow tract myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 26 heart primordium cardiac mesoderm myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 29 and 30 myocardium heart myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 35 and 36 myocardium myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 37 and 38 right lymph heart left lymph heart jaw muscle heart myh15.L myh7, vMHC X. laevis Throughout NF stage 45 left atrium right atrium heart outflow tract myh6.S aMHC, ck231, MHC, MHCa, MHC-alpha, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 45 heart cardiac ventricle left atrium right atrium cardiac mesoderm outflow tract Image source: Published Larger Image Printer Friendly View

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