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Fig. 2. Expression of Pmp22 in whole-mount in situ hybridization. (A) Expression of Pmp22 at stage 15 in the cement gland. Anterior view, dorsal to top. (B) At stage 22, Pmp22 was detected in the developing somites and profundal and trigeminal nerves. (C) At stage 22 Pmp22 sense probe was used as a negative control. (D) Pmp22 was expressed in the profundal and trigeminal ganglia, cranial mesenchyme, and somites at stage 29/30, where it was also beginning to be expressed in the branchial arches. (E) Strong expression of Pmp22 was observed in the craniofacial region. (F–G, I) To confirm the tissue-specific expression of Pmp22, we also performed in situ hybridization for Sox9 and Islet1. (H, I) At stage 35, both Pmp22 and Sox9 were detected in the branchial arches. Panels (B–I), lateral view, anterior to the left. ba, branchial arches; cg, cement gland; pr, profundal ganglia; s, somites; tg, trigeminal ganglia. Image published in: Tae HJ et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue pmp22.L cmt1a, cmt1e, dss, gas-3, hmsnia, hnpp, sp110 X. laevis Throughout NF stage 15 cement gland primordium paraxial mesoderm pmp22.L cmt1a, cmt1e, dss, gas-3, hmsnia, hnpp, sp110 X. laevis Throughout NF stage 26 somite trigeminal ganglion profundal placode presomitic mesoderm caudal presomitic mesoderm sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Sometime during NF stage 26 to NF stage 32 postmigratory neural crest cell hyoid crest mandibular crest otic vesicle brain eye branchial crest pmp22.L cmt1a, cmt1e, dss, gas-3, hmsnia, hnpp, sp110 X. laevis Throughout NF stage 28 somite trigeminal ganglion profundus ganglion pharyngeal arch mandibular arch hyoid arch branchial arch presomitic mesoderm caudal presomitic mesoderm isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 28 trigeminal ganglion pineal gland profundus ganglion olfactory placode lateral line ganglion epibranchial placode pmp22.L cmt1a, cmt1e, dss, gas-3, hmsnia, hnpp, sp110 X. laevis Throughout NF stage 35 and 36 head somite tail somite pharyngeal arch sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 35 and 36 otic vesicle brain head mesenchyme pharyngeal arch mandibular arch hyoid arch branchial arch Image source: Published Larger Image Printer Friendly View

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