Xenbase Image ID: 135689

Fig. S2. Analysis of Gtpbp2 temporal expression by quantitative RT-PCR and whole mount in situ hybridization. (A) Gtpbp2 transcripts were quantified across developmental stages, with expression levels normalized to the housekeeping gene ornithine decarboxylase (odc) and shown in arbitrary units by setting the expression level of a fertilized egg (Stage1) to 1. Gtpbp2 expression significantly decreases during gastrulation. Stage 11 corresponds to mid-gastrulation and stage 12 corresponds to late gastrulation. (B) Whole mount in situ hybridization on staged embryos, confirming the localization of gtpbp2 transcripts to the animal pole of the egg and early cleaving 4-cell blastula. Neurula stage expression is weak but enriched in neural folds at early (stage 15) and late (stage 20) neurula. Stage 25, early tailbud tadpole expression of gtpbp2 is most prominent in the somites (s) and eye (e), with enrichment in the central nervous system (cns). A sense strand RNA in situ probe was hybridized to the tadpole at top, and an antisense probe was hybridized to the tadpole at bottom. Image published in: Kirmizitas A et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gtpbp2.L GTP-binding protein 2 X. laevis Throughout NF stage 1 animal pole animal gtpbp2.L GTP-binding protein 2 X. laevis Throughout NF stage 15 pre-chordal neural plate gtpbp2.L GTP-binding protein 2 X. laevis Throughout NF stage 20 neural plate pre-chordal neural plate chordal neural plate optic vesicle gtpbp2.L GTP-binding protein 2 X. laevis Throughout NF stage 25 somite eye pharyngeal region foregut gtpbp2.L GTP-binding protein 2 X. laevis Throughout NF stage 3 (4-cell) animal pole animal blastomere animal Image source: Published Larger Image Printer Friendly View

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