Xenbase Image ID: 138582

Fig 2. rmnd5 is expressed during early embryonic development. (A) Temporal RT-PCR analysis of rmnd5 expression (top panel); different developmental stages (NF-stages) indicated at the top. ODC1 functions as RNA input control (bottom). (B) Rmnd5 protein at different developmental stages. Western blot analysis of embryo lysate from indicated stages (top). α-RMND5A (Novus Biological; rabbit, 1:1000); α-Tubulin (AbD Serotec, rat, 1:2500). (C) Spatial analysis of rmnd5 expression. Whole mount in situ hybridisation (Wmish) of wild type Xenopus laevis embryos at different developmental stages. NF stage 3 (panel a, left) and stage 4 (panel c) rmnd5 transcript in the animal pole (top), NF-stage 12 (panel d) rmnd5 transcripts around the prospective head, NF-stage 18; 24 (panel e, f, g, h) neuronal ectoderm (red arrow, panel e) and ciliated cells of the skin (yellow arrow, panel e, g, h), NF-stage 34 (panel j, k, l, m) proencephalon (red arrow) and eyes (green arrow). Negative controls with sense probes (panel b, i). Image published in: Pfirrmann T et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue rmnd5a.L rmnd5 X. laevis Throughout NF stage 18 ectoderm eye head neuroectoderm ciliated epidermal cell rmnd5a.L rmnd5 X. laevis Throughout NF stage 24 eye head somite ciliated epidermal cell neuroectoderm rmnd5a.L rmnd5 X. laevis Throughout NF stage 33 and 34 eye head forebrain cranial neural crest somite intermediate mesoderm rmnd5a.L rmnd5 X. laevis Sometime during NF stage 3 (4-cell) to NF stage 4 (8-cell) animal pole animal animal blastomere Image source: Published Larger Image Printer Friendly View

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