Xenbase Image ID: 139654

Figure S1. Related to Figure 1. Expression of Sox5 in wildtype embryos and Sox5 is effectively knocked down (A-C) In situ hybridization showing expression of Sox5 with schematics showing location (green) of NPB and premigratory and migratory NC cells at: (A) early neurula (stage 13) (B) mid-neurula (stage 18) (C) tailbud (stage 24) stages. At early neurula stages Sox5 is expressed in the somitic mesoderm and throughout the ectoderm. By stage 18 the pan-ectodermal expression has been lost and robust expression is seen at the NPB. (D-F) 60 μM serial sections through embryos following in situ hybridization for Sox5. Strong expression in mesoderm is seen at stage 13 (D) while expression in the neural crest can be seen at mid-neurula stages (E), and tailbud stages (F). (G) In situ hybridization of wildtype embryos showing Sox5 expression at the two cell (stage 2), morula (stage 6) and blastula (stage 8) stages. Maternal expression was also reported in a proteomics study (Yanai et al., 2011). (H) RT-PCR stage 9 animal pole explants, vegetal bottom explants and whole embryos Sox5 is expressed in both animal and vegetal explants whereas Mix.1 is expressed preferentially in the vegetal pole and Xema in the animal pole. Histone H4 was used to normalize cDNA input. (I) Wild type expression of Sox6 from stage 8 through stage 32. In contrast to Sox5, Sox6 lacks detectable early ectodermal/NC expression. (J) Western blot of lysates from embryos co-injected with Sox5 mRNA and Sox5 morpholino demonstrating that Sox5 translation is blocked by the morpholino. An N-terminally myc tagged Sox5 cannot be targeted by the morpholino. (K) In situ hybridization examining expression of Snail2 in embryos injected with Sox5 mRNA, Sox5 morpholino, or both. Coinjection of Sox5 mRNA rescues the effects of Sox5 morpholino. Image published in: Nordin K and LaBonne C (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox5.S XSox-5 X. laevis Throughout NF stage 13 mesoderm axial mesoderm paraxial mesoderm sox5.S XSox-5 X. laevis Throughout NF stage 18 neural plate border prechordal plate anterior placodal area cranial neural crest paraxial mesoderm presomitic mesoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 cranial neural crest sox6 LOC108713441 X. tropicalis Sometime during NF stage 18 to NF stage 23 neural tube sox5.S XSox-5 X. laevis Throughout NF stage 2 (2-cell) animal pole sox5.S XSox-5 X. laevis Throughout NF stage 25 somite cranial neural crest mandibular crest hyoid crest branchial crest anterior branchial crest posterior branchial crest notochord roof plate pharyngeal arch mandibular arch hyoid arch branchial arch sox6 LOC108713441 X. tropicalis Sometime during NF stage 28 to NF stage 32 somite spinal cord floor plate pharyngeal arch mandibular arch hyoid arch branchial arch sox5.S XSox-5 X. laevis Throughout NF stage 6 (32-cell) animal blastomere sox5.S XSox-5 X. laevis Throughout NF stage 8 animal hemisphere sox6 LOC108713441 X. tropicalis Throughout NF stage 8 Image source: Published Larger Image Printer Friendly View

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