XB-IMG-145075
Xenbase Image ID: 145075
|
Figure 4. Xsyntenin expressions during X. laevis early development
and Xsyntenin-a corecruitment with XDsh, by XFz 7, to the
plasma membrane of animal caps. (A) RNA extracts from embryos
at different stages were used to analyze Xsyntenin-a expression by
RT-PCR. Histone H4 amplification was used as an internal control.
(BâI) Xsyntenin-a, -aâ, and b and XFz 3, 7, and 8 mRNA distributions
(purple) at different stages (st) of development. Hd, head; nt, neural
tube; pn, pronephros; ba; branchial arches; cg, cement gland. (J)
Syntenin-Fz 7 interaction is conserved in Xenopus. Hu Fz7 and XFz7
cytoplasmic domain sequences are shown on the right. The sequences
vary for two amino acids (underlined). Fz 7 cytoplasmic
domain from Xenopus or human, mutated (T/A) or not mutated in
the PDZBM, was overlayed with Xsyntenin-a (top). The quality and
concentration of the fusion proteins were controlled in Coomassie
(bottom). (KâU) Confocal micrographs of Xenopus animal caps at
stage 9 showing the subcellular distribution of XDsh-myc (KâM and
Q), Xkermit-myc (S and T), Xsyntenin-a-myc (NâP) or Xsyntenina-
HA (R and U) as indicated at the bottom. The caps originate from
embryos injected at two-cell stage with different combinations of
mRNAs encoding proteins indicated on each micrograph. Note the
translocation of XDsh to the plasma membrane upon XFz 7 and
XFz 7 T/A expression (compare M and L with K) and the translocation
of Xsyntenin-a upon XFz 7 but not XFz7 T/A expression
(compare O and P with N). Note also that although Xsyntenin-a
impairs Xkermit translocation (T), XDsh translocation is maintained
(Q). Image published in: Luyten A et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published Larger Image Printer Friendly View |