Xenbase Image ID: 147689

Fig. 3. CRISPR injected embryos phenocopy established mutant or MO knockdown phenotypes in the F0 generation. Left panel=WT embryo, Middle panel=MO injected embryo, Right panel=CRISPRp injected embryo. (a) Foxj1 CRISPRp – Immunohistochemistry of stage 28 embryos with acetylated tubulin to mark surface cilia. (b) Pax8 CRISPRp – in situ hybridization of stage 38 embryos with pax2 probe to stain kidney tubule. Black arrow points to pronephros. Image published in: Bhattacharya D et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 28 cilium multiciliated epidermal cell pax2 LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. tropicalis Throughout NF stage 37 and 38 otic vesicle pronephric kidney brain midbrain-hindbrain boundary hindbrain optic stalk pronephric nephrostome Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + foxj1 CRISPR + NF28 (immunohistochemistry) fig.3.a Anatomical Phenotype abnormal ciliated epidermal cell abnormally decreased number of multiciliated epidermal cell abnormally decreased number of ciliated epidermal cell Xtr Wt + foxj1 MO + NF28 (immunohistochemistry) fig.3.a Anatomical Phenotype abnormal ciliated epidermal cell abnormally decreased number of ciliated epidermal cell abnormally decreased number of multiciliated epidermal cell Disease facial neoplasm autoimmune disease allergic rhinitis Xtr Wt + pax8 CRISPR + NF37/38 (in situ hybridization) fig.3.b Anatomical Phenotype abnormal pronephric kidney morphology abnormal development of pronephric kidney Disease kidney disease Expression Phenotype decreased amount pax2 expression in pronephric kidney Xtr Wt + pax8 MO + NF37/38 (in situ hybridization) fig.3.b Anatomical Phenotype abnormal development of pronephric kidney abnormal pronephric kidney morphology Disease kidney disease Larger Image Printer Friendly View

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