Xenbase Image ID: 148314

Fig. 2. Expression patterns of gata2, bcar3 and bcar1 during Xenopus development (A) Northern analysis showing bcar3 RNA transcripts. odc was used as a loading control. All lanes are from the same blot, aligned following removal of intervening lanes (following the 2nd and 3rd lanes) using Photoshop. N/F: Nieuwkoop and Faber stages. (B-II) WMISH analysis of expression of gata2 (B–D and M–Q), bcar3 (E–H, R–V and BB–EE) and bcar1 (I–L, W–AA and FF–II) during development. For each stage, the same embryo is shown in animal, vegetal, lateral, dorsal or ventral views. The embryos in panels CC and GG were mounted in Murray's clear to visualize internal structures. Brown is pigment. All other embryos were photographed in PBS to visualize superficial structures. (DD, EE, HH and II) Transverse sections of previously stained st. 34 embryo at the level shown in panels CC and GG respectively. Np: neural plate, n: notochord, anf: anterior neural folds, b: brain, sm: somitic mesoderm, e: eye, cg: cement gland, g: cranial ganglia, sc: spinal cord, fm: fin mesenchyme, tb: tail bud, ma: abdominal muscle anlagen, p: pronephros, c: cloaca, ba: branchial arches, vbi: ventral blood island. Image published in: Green YS et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue bcar1.L CASB3b, p130Cas X. laevis Throughout NF stage 10 animal hemisphere gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 12.5 ventral non-neural ectoderm bcar3.L and-34, nsp2, sh2d3b X. laevis Throughout NF stage 12.5 non-neural ectoderm ectoderm notochord bcar1.L CASB3b, p130Cas X. laevis Sometime during NF stage 12.5 to NF stage 20 ectoderm gata2.L GATA-2, xGATA-2, xgata2 X. laevis Sometime during NF stage 17 to NF stage 20 non-neural ectoderm ventral bcar3.L and-34, nsp2, sh2d3b X. laevis Sometime during NF stage 17 to NF stage 20 non-neural ectoderm notochord ventral pre-chordal neural plate bcar3.L and-34, nsp2, sh2d3b X. laevis Sometime during NF stage 2 (2-cell) to NF stage 3 (4-cell) animal blastomere gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 23 ventral bcar3.L and-34, nsp2, sh2d3b X. laevis Throughout NF stage 23 non-neural ectoderm optic vesicle bcar1.L CASB3b, p130Cas X. laevis Throughout NF stage 23 brain optic vesicle bcar3.L and-34, nsp2, sh2d3b X. laevis Throughout NF stage 33 and 34 notochord cement gland eye hypaxial muscle pronephric duct post-anal gut cranial nerve fin dorsal fin margin ventral fin margin spinal cord brain bcar1.L CASB3b, p130Cas X. laevis Throughout NF stage 33 and 34 cement gland notochord central nervous system brain eye spinal cord pronephric kidney pronephric duct pronephric nephron pronephric tubule pharyngeal arch midbrain hindbrain fin dorsal fin margin ventral fin margin forebrain gata2.L GATA-2, xGATA-2, xgata2 X. laevis Sometime during NF stage 8 to NF stage 10 animal hemisphere bcar3.L and-34, nsp2, sh2d3b X. laevis Sometime during NF stage 8 to NF stage 10 animal hemisphere Image source: Published Larger Image Printer Friendly View

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