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Figure 2—figure supplement 4. Validation of Rfx2-dependent genes. (A) Overview of the animal cap assay. (B) RT-PCR results. Ctl: control animal caps. Rfx2 MO: Rfx2 morpholino-injected animal caps. α-tubulin expression was not changed. ef1α served as a loading control. (C) ccdc63, ccdc104, dnal1, ribc2, ropn1l, tekt3, and ttc29 were expressed in MCCs and their expressions were Rfx2-dependent. Image published in: Chung MI et al. (2014) Copyright © 2013, Chung et al. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue ccdc63.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell cfap36.L ccdc104 X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell dnal1.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell ribc2.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell ropn1l.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell tekt3.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell ttc29.L X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell tuba8.S tubal2 X. laevis Throughout NF stage 20 ciliated epidermal cell ciliated cell Image source: Published Larger Image Printer Friendly View

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