Xenbase Image ID: 150822

Fig. S2. Temporal and spatial expression analysis of Pkdcc1/2. (A) Total RNA was obtained from Xenopus laevis embryos at different stages of development and analyzed by RT-PCR. Strong Pkdcc1/2 expression was observed from early gastrula stage (stage 10.5) until tailbud stages. Faint maternal expression was observed at 4-cell stage. Histone 4 (H4) was used as a loading control. H2O and –RT samples are used as negative controls. (B-I) Whole-mount in situ hybridization of Pkdcc1 and 2 at post-gastrula stages showed strong expression in the anterior part of the embryo at stage 13. At stage 15, expression was observed in the lateral plate mesoderm (Pkdcc1) or in the neural tube (Pkdcc2). At stage 24 and 28, robust expression was mainly found in the eye and in the lateral plate mesoderm, with Pkdcc2 lateral plate mesoderm expression being more anteriorly restricted. Abbreviations: lpm, lateral plate mesoderm; nt, neural tube; a, anterior; p, posterior. Image published in: Ding Y et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pkdcc.S pkdcc.1, pkdcc1, sgk493 X. laevis Throughout NF stage 13 anterior pkdcc.2.S pkdcc2, sgk493, vlk X. laevis Throughout NF stage 13 anterior pkdcc.S pkdcc.1, pkdcc1, sgk493 X. laevis Throughout NF stage 15 lateral plate mesoderm pkdcc.2.S pkdcc2, sgk493, vlk X. laevis Throughout NF stage 15 neural fold neural plate chordal neural plate pkdcc.S pkdcc.1, pkdcc1, sgk493 X. laevis Throughout NF stage 24 lateral plate mesoderm eye pkdcc.2.S pkdcc2, sgk493, vlk X. laevis Throughout NF stage 24 lateral plate mesoderm eye pkdcc.S pkdcc.1, pkdcc1, sgk493 X. laevis Throughout NF stage 28 eye ventral blood island pkdcc.2.S pkdcc2, sgk493, vlk X. laevis Throughout NF stage 28 eye Image source: Published Larger Image Printer Friendly View

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