Xenbase Image ID: 151758

Fig. 1. Sdc4 is required for foregut organogenesis (A) Schematic of a NF19 stage Xenopus embryo in mid-sagittal section with the foregut (fg) endoderm shown in dark grey and mid/hindgut endoderm in light grey. (B) In situ hybridization showing sdc4 expression at stage NF19. (C) Confocal image showing Alexa lineage tracer (red) in a bisected stage NF19 embryo confirming the targeted microinjection of the foregut endoderm. (D) Western blot analysis of NF19 dissected foreguts confirming Sdc4 depletion by Sdc4-MO injection. (E–G) Lateral view of stage 45 embryos showing foregut hypoplasia in Sdc4-depleted embryos that is rescued by sdc4 mRNA injection. (H–J) In situ hybridization of a2m in isolated stage 45 gut tube showing hypoplastic liver (lv), stomach (st) and pancreas (p). (K–P) In situ hybridization with liver (nr1h5) and pancreas/duodenum (pdx1) markers at stage NF35 showing defects in organ specification, which are rescued by sdc4 RNA injection. In all panels, numbers indicate the embryos exhibiting the phenotype. Image published in: Zhang Z et al. (2016) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-NoDerivatives license Gene Synonyms Species Stage(s) Tissue sdc4.S amphiglycan, ryudocan, sdc4-a, sdc4-b, syn-4, syn4, syndecan-4, Syndecan4 X. laevis Throughout NF stage 19 foregut endoderm endoderm notochord prechordal plate pdx1.L ipf1, pdx-1, STF-1, XlHbox-8, XlHbox8 X. laevis Throughout NF stage 35 and 36 pancreas duodenum nr1h5.L for1 X. laevis Throughout NF stage 35 and 36 liver a2m.L A2Moo, Alpha2-macroglobulin, edd, endod, endodermin, LOC108695707, ovomacroglobulin X. laevis Throughout NF stage 45 stomach liver foregut Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + sdc4 MO + NF45 (whole-mount microscopy) Fig 1. F. Anatomical Phenotype abnormal alimentary system morphology decreased size of the alimentary system decreased size of the foregut decreased size of the eye abnormal head morphology Xla Wt + sdc4 MO + NF45 (in situ hybridization) Fig 1. I Expression Phenotype decreased amount a2m.L expression in foregut decreased amount a2m.L expression in liver Xla Wt + sdc4 MO + NF45 (in situ hybridization) Fig 1. L Expression Phenotype decreased amount pdx1.L expression in duodenum decreased amount pdx1.L expression in pancreas Xla Wt + sdc4 MO + NF45 (in situ hybridization) Fig 1. O Expression Phenotype decreased amount nr1h5.L expression in liver Larger Image Printer Friendly View

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