Xenbase Image ID: 156908

Fig. 1. Fzd3 loss of function results in an abnormal eye development. A. Spatial expression pattern of fzd3. Fzd3 transcripts are found in the neural tube (black arrowheads), migrating neural crest cells (white arrows) and the developing eye (white arrowheads) at indicated stages. B. Loss of Fzd3 led to smaller and deformed eyes on the injected side (white arrows) in comparison to the un-injected or Control MO injected side. Furthermore, Fzd3 inhibition resulted in disturbed retinal pigmented epithelium (RPE, red arrows). Representative embryos are shown. Scale bar (sections): 100 µm. C. Quantitative representation of the data shown in B. Note, that also the data from Fig. 5B are incorporated into the statistics. D. Detailed views of Fzd3 MO and Control MO injected eyes. Dashed red lines indicate measured eye areas. Red lines indicate the eye fissure angle (°fis.) measured. Quantitative representations of eye area and eye fissure angle (°fis.) measurements are given. E. Cross sections of Fzd3-depleted embryos after WMISH using marker genes against different retinal cell types. Loss of Fzd3 led to disorganized retinal cell layers including an invagination of the photoreceptor layer into inner retina layers (black arrows) and the formation of rosette-like structures by ectopic photoreceptor cells (red arrowheads). The ratios of Fzd3 MO injected embryos showing retinal lamination defects versus all investigated embryos are indicated. Scale bar: 100 µm. F. Lens specific markers celf1 and cryba1 are shown by WMISH. Marker gene expression was unaffected upon Fzd3 depletion although some lenses were smaller (the ratios of Fzd3 MO injected embryos showing smaller lenses versus all investigated embryos are indicated). Scale bar: 100 µm. Abbreviations: GCL, ganglion cell layer; INL, inner nuclear cell layer; n, number of independent experiments; N, total number of analyzed embryos; ONL, outer nuclear cell layer; st, stage. Error bars indicate standard error of the mean (s.e.m.). **** p≤0.0001. p values were calculated by non-parametric Mann–Whitney rank sum test. Image published in: Seigfried FA et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue fzd3.L frizzled 3, frizzled-3, frizzled3, frz-3, frz3, Fz-3, fz3, Xfz3 X. laevis Throughout NF stage 20 neuroectoderm neural plate neural tube migratory neural crest cell optic vesicle fzd3.L frizzled 3, frizzled-3, frizzled3, frz-3, frz3, Fz-3, fz3, Xfz3 X. laevis Throughout NF stage 23 neuroectoderm neural tube migratory neural crest cell optic vesicle fzd3.L frizzled 3, frizzled-3, frizzled3, frz-3, frz3, Fz-3, fz3, Xfz3 X. laevis Throughout NF stage 28 head brain eye pharyngeal region central nervous system spinal cord forebrain midbrain hindbrain intermediate mesoderm otic vesicle arr3.L X. laevis Throughout NF stage 42 retina eye photoreceptor cell eye photoreceptor layer rho.L opn2, rh1, rhodopsin, rp4, xrho X. laevis Throughout NF stage 42 retina eye photoreceptor cell eye photoreceptor layer vsx1.L ktcn, ppcd, ppd, rinx, Xvsx1 X. laevis Throughout NF stage 42 retina bipolar neuron eye prox1.L prospero, prospero homeobox protein 1, prospero-related homeobox 1, Prox 1, xprox1 X. laevis Throughout NF stage 42 horizontal cell retina eye pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 42 retina amacrine cell retinal ganglion cell eye retinal ganglion cell layer pou4f1.L Brn3.0, brn3a, LOC108709765, oct-t1, POU class IV homeobox 1, rdc-1 X. laevis Throughout NF stage 42 retina retinal ganglion cell eye retinal ganglion cell layer celf1.L brunol2, celf1-a, celf1-b, cug-bp, cugbp, cug-bp1, cugbp1, eden-bp, edenbp, embryo deadenylation element binding protein, nab50 X. laevis Throughout NF stage 42 eye lens cryba1.L cryb1 X. laevis Throughout NF stage 42 eye lens Image source: Published Larger Image Printer Friendly View

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