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Figure 1. XArx is expressed in a dynamic pattern in the developing diencephalon and telencephalon. A-L: Double whole-mount in situ hybridization (WISH) using antisense riboprobes specific for xArx (blue color) and other forebrain markers (in red): Rx2A (A,B), otx1 (C), en-2 (D), FoxG1 (E-H), dlx5 (I), emx1 [note typological error- 'mex1' sic] (J), Nkx2.1 (K), and eomesodermin (L). Regions of overlap appear purple. At neural plate and neural tube stages, xArx expression is caudal/posterior to telencephalic markers such as Rx2A and FoxG1. XArx expression is rostral to otx1 and en-2. E-H: xArx is expressed caudal to FoxG1 at neural plate and neural tube stages. During tailbud stages, xArx expression spreads rostrally to overlap a portion of the FoxG1 expression domain (arrow in H). XArx expression also overlaps expression of dlx5, mex1, Nkx2.1, and eomesodermin. Anterior views are shown in A-I. Lateral views are shown in J-L.
