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XB-IMG-157990

Xenbase Image ID: 157990


Fig. 6. Nosip and Rbp1 act synergistically duringXenopusbrain development (A)rbp1-, opa1- and syne2-specific WMISH of Xenopus embryos at indicated stages; black arrowheads pointing to anterior neural plate at stage 13 and brain at stages 23 and 30, white arrowhead pointing to the eye, red arrowhead to pharyngeal arches (B)rbp1-specific WMISH of Xenopus embryos at stage 23 after unilateral injection of Nosip MO or Control MO as indicated; injected side at the right, arrowhead pointing to reduced rbp1 expression in the brain (C) Statistical evaluation of the data shown in B (D) Bright field images of Xenopus brains at stage 42, anterior to the top, after unilateral injection of Rbp1 MO or Control MO and GFP mRNA as indicated; scale bar 200 µm (E) Statistical evaluation of the data shown in D, N = number of independently evaluated brains (F) Synergy experiment. Bright field images of Xenopus brains at stage 42, after unilateral injection of either 10 ng Nosip MO or 30 ng Rbp1 MO alone or in combination. 0.5 ng GFP mRNA was co-injected as lineage tracer; scale bar 200 µm (G) Statistical evaluation of the data shown in F, N = number of independently evaluated brains.

Image published in: Hoffmeister M et al. (2017)

Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
rbp1.LCRBP1X. laevisThroughout NF stage 13neural plate
opa1.LX. laevisThroughout NF stage 13pre-chordal neural plate
syne2.Ledmd5, LOC108698815, nesprin-2, nua, nuance, syne-2X. laevisThroughout NF stage 13pre-chordal neural plate
rbp1.LCRBP1X. laevisThroughout NF stage 23anterior neural tube
opa1.LX. laevisThroughout NF stage 23anterior neural tube
optic vesicle
syne2.Ledmd5, LOC108698815, nesprin-2, nua, nuance, syne-2X. laevisThroughout NF stage 23anterior neural tube
rbp1.LCRBP1X. laevisThroughout NF stage 29 and 30brain
midbrain
eye
opa1.LX. laevisThroughout NF stage 29 and 30brain
central nervous system
spinal cord
eye
pharyngeal arch
somite
syne2.Ledmd5, LOC108698815, nesprin-2, nua, nuance, syne-2X. laevisThroughout NF stage 29 and 30brain
central nervous system
eye

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