Xenbase Image ID: 158188

Figure S1. Whole mount in situ hybridization (WISH) of select vegetal and animal pole transcripts. Expression of a subset of RNAs enriched in the vegetal (A-B) and animal (C) poles were analyzed during oogenesis and development by WISH. Probes, developmental stages, and developmental structures are indicated: germplasm/PGCs (purple), pronephros (pink), ventral blood islands (lime green), eye (black), lens (white), otic vesicle (gray), cranial ganglia (yellow), brachial arches (green), nasal placodes (teal), inter-segmental region (brown), notochord (orange), brain and neural tube (blue). Transcripts detected in primordial germ cells at the tailbud stage are magnified and embedded in their respective images. Black bars represent 100um in st. I oocyte panels, and 200um in all other panels. Please refer to Fig. 3 for use of xpat probe as a positive control for germ plasm localization. Image published in: Owens DA et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue atrx.L LOC108700535 X. laevis Throughout NF stage 16 primordial germ cell hook2.L hk2, LOC108703487 X. laevis Throughout NF stage 16 primordial germ cell tob2.L tob4, tobl, trob2 X. laevis Throughout NF stage 16 primordial germ cell mesoderm rnf38.L X. laevis Throughout NF stage 16 primordial germ cell neural plate trank1.L X. laevis Throughout NF stage 16 primordial germ cell atrx.L LOC108700535 X. laevis Throughout NF stage 32 eye central nervous system brain spinal cord hindbrain hook2.L hk2, LOC108703487 X. laevis Throughout NF stage 32 eye central nervous system brain spinal cord tob2.L tob4, tobl, trob2 X. laevis Throughout NF stage 32 somite eye pineal gland rnf38.L X. laevis Throughout NF stage 32 eye central nervous system spinal cord hindbrain parn.L xparn X. laevis Throughout NF stage 32 brain head pharyngeal region wwtr1.L taz, xtTAZ X. laevis Throughout NF stage 32 spinal cord pharyngeal region heart myoblast blood vessel atrx.L LOC108700535 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere hook2.L hk2, LOC108703487 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere tob2.L tob4, tobl, trob2 X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere rnf38.L X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere parn.L xparn X. laevis Throughout NF stage 6 (32-cell) blastomere dand5.L 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco X. laevis Throughout NF stage 6 (32-cell) animal blastomere animal animal hemisphere slc18a2.L LOC108712709, vmat2 X. laevis Throughout NF stage 6 (32-cell) animal blastomere animal trank1.L X. laevis Throughout NF stage 6 (32-cell) vegetal blastomere trank1.L X. laevis Throughout oocyte stage I mitochondrial cloud vegetal cortex rnf38.L X. laevis Sometime during oocyte stage I to oocyte stage IV vegetal pole Image source: Published Larger Image Printer Friendly View

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