Xenbase Image ID: 159615

Figure 5. Effect of Vismodegib treatment on Mauthner Cell Development. A, 3A10 antibody labeling of a pair of Mauthner cells from a control Xenopus. B, 3A10 antibody labeling of a pair of Mauthner cells from an animal treated with 6.25 µM Vismodegib. Arrowheads for A and B indicate axon crossing at the midline. C, 3D reconstruction of a control Mauthner cell from dextran amine labeling. D, 3D reconstruction of a Mauthner cell from an animal treated with 6.25 µM Vismodegib. E, Shh inhibition results in a significant reduction in the degree of branching of the Mauthner cell in 6.25 µM Vismodegib-treated animals (n = 4) compared with control animals (n = 4). F, Shh inhibition results in an approximately two-fold reduction in axonal diameter of the Mauthner cell in 6.25 µM Vismodegib-treated animals (n = 3) compared with control animals (n = 5). Scale bars represent 100 µm.* p < 0.05, ****p < 0.0001. [Color figure can be viewed at wileyonlinelibrary.com] Image published in: Zarei S et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xla Wt + Vismodegib + NF44 (immunohistochemistry) Figure 5 D Anatomical Phenotype abnormal Mauthner cell Xla Wt + Vismodegib + NF44 (immunohistochemistry) Figure 5 E Anatomical Phenotype abnormal Mauthner cell morphology Xla Wt + Vismodegib + NF44 (immunohistochemistry) Figure 5 F Anatomical Phenotype decreased size of the Mauthner cell Larger Image Printer Friendly View

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