Xenbase Image ID: 171019

Fig. 3. PR130 depletion affects somite organization and tail development. (A) RT- PCR analysis of X. tropicalis total RNA from different developmental stages. Xpr130 is expressed maternally (stage 7) and throughout early development (stages 10, 18, 22, and 30). RT, RT-PCR without reverse transcriptase. ODC1 expression was used as loading control. (A Lower ) Whole-mount ISH for Xpr130 in embryos showing predominant staining of somites from the earliest stage detectable. (B) ISH of tailbud stage embryo with an Xnkd-specific probe. (C) Control embryo shows organized somites in a chevron-like shape as visualized by whole-mount ISH by using XmyoD as a marker (Left). PR130-depleted em- bryos lack chevron-like shaped somites (Right). Arrowheads show somitic borders. (D) Similar to B but without XmyoD. Shown are embryos injected with control MO (CoMO) (Left), PR130 MO (Center), and PR130 MO plus hPR130 RNA (Right). (Lower) Sagittal sections of the somites. Image published in: Creyghton MP et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue ppp2r3a PR130, PR72 X. tropicalis Sometime during NF stage 19 to NF stage 20 somite nkd1 Naked cuticle, nkd X. tropicalis Throughout NF stage 22 to NF stage 44 somite myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. tropicalis Throughout NF stage 22 to NF stage 44 somite mouth paraxial mesoderm head ppp2r3a PR130, PR72 X. tropicalis Sometime during NF stage 23 to NF stage 24 somite ppp2r3a PR130, PR72 X. tropicalis Throughout NF stage 26 somite ppp2r3a PR130, PR72 X. tropicalis Throughout NF stage 37 and 38 somite Image source: Published Larger Image Printer Friendly View

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