Xenbase Image ID: 171984

Figure S4 Expression pattern of XFLRT2 mRNA during Xenopus laevis development. a-e, Whole-mount in situ hybridization of XFLRT2. Expression is first seen anteriorly at early neurula stages (a, stage 14, anterior view). b, Stage 18, animal view. c, Stage 24, lateral view. XFLRT2 is predominantly expressed in the head. At tailbud stages, stage 32 (d) and stage 36 (e), XFLRT2 transcripts are detected in the head and in the somites. f, RT-PCR analysis of XFLRT3 and XFLRT2 expression in embryos of the indicated stages. -RT, minus reverse transcription control sample; H4, histone H4 for normalization. g, Sequence of the FLRT2 morpholino target region. An alignment of Xenopus laevis (X.l. FLRT2) and tropicalis (X. trop. FLRT2) FLRT2 nucleotide sequences is shown. A XFLRT2 pseudoallel was also identified (not shown) whose sequence is identical in the XF2(b)-MO target region but has two base-pair mismatches in the XF2(a)-MO target region. Image published in: Böttcher RT et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Gene Synonyms Species Stage(s) Tissue flrt2.L X. laevis Throughout NF stage 14 anterior neural ridge flrt2.L X. laevis Throughout NF stage 18 anterior neural ridge flrt2.L X. laevis Throughout NF stage 24 head posterior mandibular crest flrt2.L X. laevis Throughout NF stage 28 cement gland hindbrain hyoid arch proctodeum pineal gland flrt2.L X. laevis Sometime during NF stage 32 to NF stage 40 hindbrain duct of Cuvier musculature of face Image source: Published Larger Image Printer Friendly View

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