XB-IMG-172738
Xenbase Image ID: 172738
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Figure 6. STRAP knockdown influences anteriorâposterior neural patterning and impairs the body axis and eye deâ
velopment in early Xenopus embryogenesis.  A. Strap transcript was detected in the entire blastopore (a), early neuâ
rulation (b), late neurula stage (c), and the tailbud and the tadpole stages (d and e, respectively). B. 50 ng StrapâMO
was injected into the animal regions of twoâcellâstage embryos and resulted in defects in the tadpole stage. C. Strap
RNA (0.20 ng) was injected alone or with 25 ng StrapâMO unilaterally into twoâcellâ stage embryos. The embryos were
stained with the βâGal substrate RedâGal. D. 50 ng StrapâMO was injected unilaterally into twoâcellâ stage embryos.
The embryos were examined at neurula stages 17â18 by ISH for neural markers. E.  50 ng StrapâMO was injected into
the animal regions of twoâcellâstage embryos. Animal caps were dissected at blastula stage 9 and cultured until neuâ
rula stages 19â20. RNA was then extracted for RTâqPCR analysis of marker expression. Bars represent mean ±s.d., n=3.
* P<0.05,  **P<0.01, when compared with the control. Image published in: Jin L et al. (2018) Copyright © 2018. OA ARTICLE, images redisplayed under a Creative Commons license.
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