Xenbase Image ID: 173126

Fig. 1 Visualization of paired-type homeobox gene expression in the developing head skeleton of tailbud stage Xenopus laevis embryos. Whole-mount in situ hybridization was performed on embryos fixed at stages 28–30 (panels a–d) or 34–36 (panels e–h) using antisense riboprobes for alx1 (a, e), alx4 (b, f), prrx1 (c, g), or prrx2 (d, h). Specific stages are indicated in the lower right corner of each panel. Gene-specific antisense riboprobes were synthesized by in vitro transcription (Ambion MEGAscript kit) using specific cDNAs generated by 3′-RACE (Clontech SMARTer RACE Kit) using total RNA isolated from mixed neurula stage embryos using degenerate primers encoding VQVWFQN (5′-GTN CAR GTN TGG TTY CAR AAY-3′). Anatomical features are indicated in panel G: C—cement gland; E—eye; F—frontonasal process; Op—olfactory placode/pit; Ov—otic vesicle; P—pharyngeal arch Image published in: El-Hodiri HM and Kelly LE (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Springer Nature. Copyright Springer Nature. Gene Synonyms Species Stage(s) Tissue alx4.L fnd2, LOC108713752, LOC108715264 X. laevis Throughout NF stage 28 prrx1.L LOC108714489, LOC108715742, phox1, pmx1, prx-1, prx1, xprx-1 X. laevis Throughout NF stage 29 and 30 alx1.L Cart-1, cart1, Xcart1 X. laevis Throughout NF stage 29 and 30 prrx2.L X. laevis Throughout NF stage 29 and 30 prrx1.L LOC108714489, LOC108715742, phox1, pmx1, prx-1, prx1, xprx-1 X. laevis Throughout NF stage 33 and 34 alx1.L Cart-1, cart1, Xcart1 X. laevis Throughout NF stage 33 and 34 alx4.L fnd2, LOC108713752, LOC108715264 X. laevis Throughout NF stage 35 and 36 prrx2.L X. laevis Throughout NF stage 35 and 36 Image source: Published Larger Image Printer Friendly View

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