Xenbase Image ID: 173164

Figure S6: Expression Analysis of G-Protein Beta Subunits. (A) Quantification of the expression levels of G-protein beta subunits of stage 39 Xenopus tropicalis embryos using RNAseq. (B) Analysis of Gnb1-MO knockdown efficacy by in vitro transcription/translation. (CG’) Whole mount in situ hybridization of Xenopus laevis embryos and paraplast sections thereof demonstrating the expression of G-protein beta subunits in stage 35 embryos. Insets show close-ups of the pronephric kidney. The expression of Gnb1 in the pronephric kidney was the most pronounced of all the G-Protein beta subunits and was, thus, selected for the knockdown studies. Image published in: Zhang B et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gnb1.S XGB1, XGbeta1 X. laevis Throughout NF stage 35 and 36 pronephric kidney spinal cord brain forebrain tail bud pharyngeal region head gnb3.S X. laevis Throughout NF stage 35 and 36 pineal gland cement gland rack1.L gnb2l1, Gnb2l1, gnb2-rs1, h12.3, hlc-7, pig21 X. laevis Throughout NF stage 35 and 36 head gnb5.L LOC108711385 X. laevis Throughout NF stage 35 and 36 pronephric kidney brain head pharyngeal region Image source: Published Larger Image Printer Friendly View

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