Xenbase Image ID: 190644

Figure S7: AER cells are largely found as cuboidal monolayer cells showing apical-basal polarity. AER cells were visualized during limb development (a) and at 5 dpa (b) in regeneration- competent tadpoles by labelling Fgf8.L mRNA. AER cells are largely present as monolayer cuboidal basal epidermal cells with apical-basal polarity. A simple squamous layer is present above AER cells, and cells with mesenchymal morphology are located underneath AER cells. From the proximal to distal midline of the epidermis, Lgr5.S expression is first detected, followed by Fgf8.L mRNA expression. Both Fgf8.L and Lgr5.S are expressed at high levels at the tip of limbs. AER cells show similar cuboidal morphology during development and regeneration. Basal epidermal cells are morphologically similar based on Hoechst and membrane markers, and Fgf8.L detection is required to detect AER cell. Row 1: Blue, Hoechst; Yellow, Fgf8.L mRNA; Red, TP63; Green, B-catenin. Row 2: Blue, Hoechst; Yellow, Fgf8.L mRNA; Red, E-Cadherin; Green, Lgr5.S mRNA. Row 3: Blue, Hoechst; Yellow, Fgf8.L mRNA; Red, E-Cadherin. Row 4: Blue, Hoechst; Yellow, Fgf8.L mRNA; Red, ITGB1; Green, Lgr5.S mRNA. Scale bars = 10 μm Image published in: Aztekin C et al. (2021) Image downloaded from an Open Access article in PubMed Central. © 2021. Published by The Company of Biologists Ltd Gene Synonyms Species Stage(s) Tissue ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 50 to NF stage 52 apical ectodermal ridge hindlimb bud epidermis tp63.L ket, np63, p63, tp73l, trp63, Xp63 X. laevis Sometime during NF stage 50 to NF stage 52 apical ectodermal ridge hindlimb bud epidermis cdh1.L arc-1, cd324, cdhe, ecad, e-cadherin, lcam, uvo, uvomorulin, XTCAD-1 X. laevis Sometime during NF stage 50 to NF stage 52 apical ectodermal ridge distal mesenchyme hindlimb bud fgf8.L AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Sometime during NF stage 50 to NF stage 52 apical ectodermal ridge hindlimb bud lgr5.S X. laevis Sometime during NF stage 50 to NF stage 52 apical ectodermal ridge hindlimb bud epidermis itgb1.L Beta 1 integrin, cd29, fnrb, gpiia, itgb1-a, itgb1-b, mdf2, msk12, vlab, vla-beta X. laevis Sometime during NF stage 52 to NF stage 54 epidermis apical ectodermal ridge regenerating hindlimb Image source: Published Larger Image Printer Friendly View

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