Xenbase Image ID: 191273

Supplementary Figure 4: RNA-Seq analysis of ift80 and ift172 targeted embryos. (f) A table of respective associations and references thereof. (g) In situ hybridization for differentially expressed genes in the RNA-Seq screen. Col1a1 expression is shown for X. laevis, all others for X. tropicalis tadpoles. (h, i) In situ hybridization of tissue marker genes was examined in X. tropicalis (h) and X. laevis (i) after unilateral CRISPR targeting of ift80 and ift172. The quantifications indicate a stronger (gray) or weaker (black) signal on the injected side. No obvious changes were found, only the snail expression, marking cranial neural crest streams, appeared to be fused in ift80 and ift172 targeted embryos. Image published in: Getwan M et al. (2021) Copyright © 2021. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-2.L nkx2.2, XeNK-2, Xnkx2.2 X. laevis Throughout NF stage 19 neural tube brain snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 23 cranial neural crest nkx2-2 nkx2.2, XeNK-2, Xnkx2.2 X. tropicalis Throughout NF stage 23 neural tube snai1.L LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 25 cranial neural crest ccng1 MGC53060 X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 head eye cfh armd4, arms1, cfhl3, fhl1, hf1, hf2, hus, MGC68456 X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 trunk dscaml1 LOC108696466, LOC108697593 X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 head olfm4 tiarin X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 proctodeum pharyngeal region riok3 sudd X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 head col1a1.L X. laevis Sometime during NF stage 29 and 30 to NF stage 40 epidermis head trunk ulk1 X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 head cacng1 X. tropicalis Sometime during NF stage 29 and 30 to NF stage 40 somite presomitic mesoderm twist1 twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. tropicalis Throughout NF stage 39 pharyngeal region twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 39 pharyngeal region Image source: Published Larger Image Printer Friendly View

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