Xenbase Image ID: 200508

Figure 1. FXR1 forms SDS-resistant amyloid-like aggregates in the brains of Xenopus laevis, Trachemys scripta, and Gallus gallus domesticus. (A) FXR1 is predominantly found as insoluble aggregates in all vertebrate species studied. Total protein lysate from the brain was treated with 1% SDS and separated into three fractions: of monomers less than 100kDa (M), oligomers larger than 100kDa (O), and insoluble aggregates (A). The fractions were subjected to SDS-PAGE and analyzed by immunoblotting with anti-FXR1 antibodies. (B) Relative intensity of bands corresponding to FXR1 monomers, oligomers, and insoluble aggregates is represented as meanSEM (standard error of the mean) for three independent brain samples for each organism. The green, yellow, and orange columns of the diagram represent the values for monomers, oligomers, and insoluble aggregates, respectively. Image published in: Velizhanina ME and Galkin AP (2022) Copyright © 2022. Image reproduced with permission of the Publisher. Larger Image Printer Friendly View

Return to previous page