Xenbase Image ID: 23816

Fig. 3. Whole mount in situ hybridization analysis of xlr expression in Xenopus embryos. (A) Mid-gastrulae (stage 11.5) viewed from the vegetal pole (AP), dorsal surface uppermost. Transcripts are localized to the lateral and ventral marginal zone and a dorsal-anterior (ant) patch. (B) Neurula stage embryo (stage 18) showing strong expression of xlr around the blastopore (post) and an anterior-ventral (ant) patch close to the cement gland. Weaker expression is also detected throughout the ventral half of the embryo. (C) Sagital section through neurula stage embryo (stage 18) showing that the strong anterior-ventral expression of xlr is localized to the pharyngeal endoderm (pe), while the weaker ventral expression is localized to the ventral mesoderm (vm). (D) Early tailbud stage embryo (stage 26) showing strong expression of xlr in the tailbud (tb) and posterior neural plate. Expression is also detected in the proctodeum (pr) and hindbrain (hb). (E) Tailbud stage embryo (stage 32) showing strong expression of xlr in the tailbud (tb) and hindbrain (hb). Note that hindbrain expression spreads into anterior portions of the spinal cord. Weaker expression can also be seen in the head, heart (he), and pronephric duct (pnd). (F–I) Transverse histological sections through a stage 32 embryo, similar to that shown in (E), showing xlr expression in the neural tube (nt). no, notochord; so, somite; en, endoderm. (F) Section through the anterior hindbrain. (G) Section through the posterior hindbrain/anterior spinal cord. (H) Section through the posterior spinal cord. (I) Section through the posterior tail. Image published in: Dale L et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tll1 Tll, Xlr, Xolloid-like, xolloid-like metalloprotease, Xolloid-related Xenopus Sometime during NF stage 10.5 to NF stage 11 dorsal anterior ventro-lateral marginal zone dorsal marginal zone tll1 Tll, Xlr, Xolloid-like, xolloid-like metalloprotease, Xolloid-related Xenopus Throughout NF stage 22 to NF stage 44 head tail bud neural tube anterior neural tube tll1 Tll, Xlr, Xolloid-like, xolloid-like metalloprotease, Xolloid-related Xenopus Throughout NF stage 27 head tail bud neural tube proctodeum hindbrain tll1 Tll, Xlr, Xolloid-like, xolloid-like metalloprotease, Xolloid-related Xenopus Throughout NF stage 32 otic vesicle hindbrain spinal cord retina heart primordium pronephric duct central nervous system neural tube Image source: Published Larger Image Printer Friendly View

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