Xenbase Image ID: 2918

Figure 9. Immunostaining of activated Smads across the dorsoventral axis. Immunostaining of activated Smads across the dorsoventral axis. Stage 10+ embryos were fixed and bisected for immunohistochemistry. Dorsoventral orientation was determined by the dorsal blastopore lip, which is indicated with an arrow in (A-D). Embryo diameter is approximately 1 mm. Immunostaining with anti-phosphoSmad1 antibody was carried out with uninjected embryos (A) or embryos injected marginally with noggin (100 pg/embryo) (B) or BMP4 (500 pg/embryo) (C). AntiphosphoSmad2 immunostaining was performed with uninjected embryos (D) or embryos injected marginally with delta1XAR1 (1 ng/embryo) (E) or activin (50 pg/embryo) (F). Image published in: Faure S et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue smad1.L MLP, smad-1, smad1-a, smad1-b, XMad, Xmad1, Xsmad1 X. laevis Throughout NF stage 10.5 mesoderm endoderm ventral ventral marginal zone vegetal yolk mass smad2.S smad-2, XSmad2 X. laevis Throughout NF stage 10.5 mesoderm upper blastopore lip endomesoderm endoderm involuting marginal zone dorsal ventral vegetal yolk mass Image source: Community submitted Larger Image Printer Friendly View

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