XB-IMG-42883
Xenbase Image ID: 42883
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Fig. 3. Whole-mount in situ hybridization analysis of XRnd1 and XRhoA transcripts. (A-I) XRnd1, (J-O) XRhoA. (A,C,K,L) Dorsal is right. (D,F,H,I,M,N,O) Anterior is left. (A) Animal (left) and vegetal (right) view of early gastrula (stage 10) embryos. XRnd1is expressed
in the ectoderm and dorsal blastopore lip. The arrowhead indicates the location of the dorsal blastopore lip. (B) Vegetal view of a midgastrula (stage 11) embryo. Note the high level of XRnd1
expression around the blastopore (arrow). (C) Mid-gastrula (stage
10.5) embryo that was cut along the midline. The arrowhead indicates the location of the dorsal blastopore. XRnd1 expression persists in the involuted mesoderm. (D) Dorsal view of an early
neurula (stage 13) embryo. XRnd1 expression is absent from the midline. (E) Histological cross section of the same embryo as in D, which reveals high level of XRnd1 expression in the somitogenic
mesoderm (SM), but not in the notochord (No). (F) Parasagittally cut midneurula (stage 20) embryo showing the high level of XRnd1 expression in unsegmented somitogenic mesoderm and expression in
newly formed somites (arrowheads). (G) Anterior transverse section of a mid-neurula stage embryo (stage 20) revealing XRnd1 expression in cranial neural crest cells (NC) and somites (So).
(H) Dorsal view of an early tailbud stage (stage 22) embryo. Note XRnd1 expression in newly formed somites (arrows) and cranial neural crest cells (arrowhead). (I) Dorsolateral view of a tailbud stage (stage 27) embryo. XRnd1 expression now remains only in the posterior region of the somitogenic mesoderm (So). (J) XRhoA expression: vegetal (left) and animal (right) view of gastrula (stage 11) embryos. XRhoA expression is present in the ectoderm and marginal zone. (K,L) two halves of a gastrula (stage 10.5) embryo
probed for XRhoA (K) and cerberus (L) to demonstrate the presence of XRhoA expression in the organizer region. Note that embryos were cut along the midline prior to in situ hybridization analysis. The
image of the embryo in K was horizontally flipped to allow for easier comparison with L. Small white arrows indicate the boundary between involuted mesoderm and overlying ectoderm. The black arrowhead shows the position of the dorsal blastopore lip and the
small arrow indicates the ventral blastopore lip. Note that both cerberus and RhoA are expressed in the ventral marginal region. XRhoA is also expressed in the ectoderm, although the surface staining appears weaker in K due to lighting conditions used for
photography. (M) Sagittally sectioned neurula embryo (stage 13) showing the expression of XRhoA in the ectoderm and mesoderm. (N) Enlargement of the boxed area in M; Me, mesoderm; Ec, ectoderm. (O) Tailbud stage (stage 23) embryo. XRhoA expression is enriched in the head and to some extent in the tail region. Image published in: Wünnenberg-Stapleton K et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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