Xenbase Image ID: 43343

Figure 3. Characterization of aqp-3b expression by whole mount in situ hybridization. aqp-3b is expressed at the edges of the neural plate in early neurula stage 13 embryos (A). As the neural folds begin to rise in stage 14+ embryos, aqp-3b expression extends more posteriorly (B). As the neural folds continue to rise and narrow the gap between them, this is reflected in aqp-3b expression in mid-neurula stage 16 and 17 embryos (D, E). At stage 18, aqp-3b expression almost meets at the midline as the neural folds have nearly come together (G). In late neurula stage 19 embryos, aqp-3b is expressed along the dorsal midline (H). The expression patterns of aqp-3b (B, E, H) are very similar to the neural fold expression of NFPC (C, F, I), while aqp-3b does not share the epidermal ectoderm expression domain of NFPC. During neurulation, aqp-3b (A, E) expression overlaps extensively with zic1 expression (J, K). Transverse sections through stained stage 18 embryos confirmed that aqp-3b is expressed at the tips of the neural folds (L). np, neural plate; nc, notochord; s, somites. Image published in: Cornish EJ et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue aqp3.L aqp-3, aqp3-a, aqp3-b, aqp-3b, aqp3b, AQP-x3, Aquaporin-3, aquaporin-3b X. laevis Sometime during NF stage 13 to NF stage 14 neural plate border zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Sometime during NF stage 13 to NF stage 17 neural plate cranial neural crest pcdh7.L nfpc X. laevis Sometime during NF stage 14 to NF stage 19 neural plate border pre-chordal neural plate border chordal neural plate border non-neural ectoderm aqp3.L aqp-3, aqp3-a, aqp3-b, aqp-3b, aqp3b, AQP-x3, Aquaporin-3, aquaporin-3b X. laevis Sometime during NF stage 16 to NF stage 18 anterior neural fold preplacodal ectoderm neural plate border pre-chordal neural plate border chordal neural plate border aqp3.L aqp-3, aqp3-a, aqp3-b, aqp-3b, aqp3b, AQP-x3, Aquaporin-3, aquaporin-3b X. laevis Throughout NF stage 19 neural crest roof plate neural tube Image source: Published Larger Image Printer Friendly View

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