Xenbase Image ID: 45231

Fig. 2. Inhibition of miRNA biogenesis affects patterning of the pronephros. (A-G′) Whole-mount in situ hybridization for markers of terminal pronephros differentiation on uninjected and Dgcr8-MO-injected Xenopus embryos at stage 39. (A,A′) SGLT1-K; (B,B′) NKCC2; (C,C′) NBC1; (D,D′) ClC-K; (E,E′) Cadherin-16; (F,F′) ROMK; (G,G′) NCC. Arrowheads indicate a reduction in the intermediate and distal tubular domain. (H,I) Schematics illustrating the different pronephric regions and their corresponding marker gene expression (H) and the phenotype of Dicer-MO- or Dgcr8-MO-injected embryos showing a reduction in the proximal and intermediate tubules as well as in distal tubule DT1 (I). Image published in: Agrawal R et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue slc5a1.2.S D22S675, sglt-1, sglt1, sglt-1k X. laevis Throughout NF stage 39 early proximal tubule late proximal tubule slc12a1.L Na-K-2Cl cotransporter, NKCC-2, NKCC2, XNKCC2 X. laevis Throughout NF stage 39 early distal tubule late distal tubule slc4a4.L hhNMC, hnbc1, nbc1, nbc2, nbce1, pNBC, slc4a5, XNBC1 X. laevis Throughout NF stage 39 early distal tubule late distal tubule clcnkb.L ClC-k, clc-kb, clckb, clcnka, clk-k2, LOC108703568, xClC-k X. laevis Throughout NF stage 39 early distal tubule late distal tubule pronephric duct cdh16.L cadherin-16, KSP-cadherin X. laevis Throughout NF stage 39 early distal tubule late distal tubule kcnj1.L kir1.1, MGC68935, ROMK, romk1 X. laevis Throughout NF stage 39 early distal tubule pronephric duct kidney slc12a3.L LOC108714020, ncc, ncct, tsc X. laevis Throughout NF stage 39 early distal tubule late distal tubule pronephric duct Image source: Published Larger Image Printer Friendly View

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