Xenbase Image ID: 46930

Figure 2. Expression Analysis of Xenopus and Mouse R-spondins(A) Rspo2 expression during Xenopus development at the indicated embryonic stages analyzed by RT-PCR. Histone H4 was used for normalization. −RT, minus reverse transcription control.(B–H) Xenopus whole-mount in situ hybridizations of the indicated genes.(B) Stage 11 embryo, dorso-vegetal view; dbl, dorsal blastoporal lip.(C) Stage 12 embryo, dorsal view with anterior up. An anterior neural expression domain is indicated by arrowhead.(D) Stage 15 embryo, dorsal view with anterior up.(E) Stage 14 embryo, dorsal view with anterior up.(F and G) Tailbud stage embryos; ba, branchial arches; cm, cranial musculature; di, diencephalon; dnt, dorsal neural tube; mhb, midbrain-hindbrain boundary; ov, otic vesicle; pn, pronephros; pdm, proctodeum; s, somites; tb, tailbud mesoderm. Inset in (F) shows a transverse section at the level indicated by arrowhead, showing expression in dorsal neural tube and in the dorsal- and ventral-most parts of the somites.(H) Dissected Xenopus tadpole brain (lateral view) showing expression in diencephalons (di) and zona limitans intrathalamica (zli), where sonic hedgehog is expressed (inset). dt, vt, dorsal and ventral thalamus, respectively; sc, spinal cord; tel, telencephalon.(I–M) Mouse whole-mount in situ hybridizations of the indicated genes.(I) Limb buds of day 12.5 mouse embryos. AER, apical ectodermal ridge.(J) Day 7.5 mouse embryo showing Rspo3 expression in the primitive streak.(K–M) Day 9.5 mouse embryos. di, diencephalon; dnt, dorsal neural tube; met, metencephalon; tel, telencephalon. Image published in: Kazanskaya O et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 11 deep superficial ventro-lateral marginal zone ectoderm rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 12 ventro-lateral ectoderm neuroectoderm anterior neural ridge ventro-lateral marginal zone rspo3 X. tropicalis Throughout NF stage 14 pre-chordal neural plate mesoderm pre-chordal neural plate border circumblastoporal collar rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 15 neural fold anterior neural fold Rohon-Beard neuron anterior placodal area ectoderm neuroectoderm rspo3 X. tropicalis Throughout NF stage 22 to NF stage 44 central nervous system branchial arch tail bud rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 29 and 30 brain diencephalon midbrain-hindbrain boundary neural tube dorsal tail fin proctodeum midbrain hindbrain post-anal gut tail bud pronephric mesenchyme pronephric duct mandibular arch central nervous system rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 50 brain diencephalon zona limitans intrathalamica shh.L hedgehog, vhh-1, Xhh, X-SHH, xshh X. laevis Throughout NF stage 50 brain neural tube zona limitans intrathalamica thalamus Image source: Published Larger Image Printer Friendly View

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