Xenbase Image ID: 47065

FIG. 2. Xlefty expression during Xenopus development. (A) Whole-mount in situ localization of Xlefty transcripts at gastrula through tailbud stages. (A) Vegetal pole view, dorsal at top. (B, C, E, J, L, and N) Dorsal view, anterior at left. (D, F, G, K, M, O, P) Left lateral view, anterior at left. (C, D), (E, F), (I, J), and (M, N) show a dorsal and a left lateral view of the same embryo. Stage of embryo(s) depicted is shown at the bottom right. (A) During early gastrulation, Xlefty expression predominantly localized to the dorsal blastopore margin. Note the punctate expression in other regions around the blastopore (arrowheads). (B) As gastrulation proceeded, Xlefty expression became localized to the prospective floor plate and prechordal plate, but was still present around the closing blastopore (arrowheads). (C, D) During early neurulation, expression continued to be localized to the prospective floor plate and prechordal plate (arrows), in addition to expression around the ventrolateral yolk plug (arrowhead). (E, F) As neurulation proceeded, the dorsal midline expression of Xlefty was downregulated centrally (arrows), resulting in an anterior and posterior domain of expression (arrowheads). (G) Following neurulation, Xlefty expression appeared in the posterior midline in the floor plate (arrowhead), notochord (asterisk), and hypochord (arrow). (H) The expression in the floor plate and hypochord remained through tailbud stages (H), while the notochord expression was transient and variable in intensity (G). The floor plate and hypochord expression progressed in a posterior-to-anterior direction (G) and receded in the opposite direction (P; data not shown). At st. 23, unilateral Xlefty expression became apparent in the left lateral plate mesoderm (arrowhead in H), then migrated anteriorly (I), and eventually became localized to the left cardiac field (arrowhead in O). Between st. 24 and st. 25, another unilateral domain of Xlefty expression emerged in the left anterior dorsal endoderm (arrowheads in K, L) and remained into early tailbud stages (K). Variable levels of bilateral posterior endoderm expression (arrow in K) were also present from approximately st. 245 through tailbud stages (K; data not shown). Image published in: Branford WW et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Sometime during NF stage 10 to NF stage 11 dorsal blastopore lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Throughout NF stage 12.5 floor plate prechordal plate blastopore lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Throughout NF stage 14 floor plate prechordal plate ventro-lateral endoderm vegetal yolk mass lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Throughout NF stage 18 anterior posterior lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Sometime during NF stage 21 to NF stage 24 notochord lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Throughout NF stage 23 lateral plate mesoderm anterior lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Sometime during NF stage 23 to NF stage 32 floor plate hypochord lefty1.L antivin, atv, leftb, lefty, lefty-1, lefty-a, lefty-b, leftyb, XATV, Xlefty X. laevis Sometime during NF stage 24 to NF stage 29 and 30 anterior dorsal endoderm posterior Image source: Published Larger Image Printer Friendly View

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