Xenbase Image ID: 47066

FIG. 3. Spatial distribution of Xbra and Xbra3 mRNA. Embryos were hybridised with antisense RNA probes representing regions of the two transcripts which differed sufficiently to ensure specificity for the two gene products (see text). At stage 12, Xbra3 (A, arrowed) is detected weakly around the blastopore in a pattern similar to that seen with Xbra (B). While Xbra message levels decline at stages 25 (C, arrowed) and 35 (D), Xbra3 is maintained at these stages in notochord and tail tip (C, D, arrowed). Hybridisation with an Xbra3 sense transcript gave no signal (C, D, control). A specific antiserum which recognises Xbra3 and not Xbra protein stains the nuclei of notochord cells at stage 35 (E, arrowed). No staining was seen with preimmune serum (not shown). Image published in: Strong CF et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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