Xenbase Image ID: 47937

Fig. 1. Expression of Nkx6 genes in X. laevis. (A–E) Whole mount in situ hybridization showing Nkx6.1 expression. (A) Nkx6.1 is not expressed during gastrulation. (B) Expression begins at stage 13 in two lateral stripes (red arrowhead) and intensifies at stage 14 (C). At stage 17 (D) Nkx6.1 expression marks where Hb9-positive MNs will form (compare to (M). (E) At stage 34, Nkx6.1 is expressed in the ventral spinal cord and hindbrain as well as the foregut (red arrowhead). (F–J) Whole mount in situ hybridization showing Nkx6.2 expression. (F) Nkx6.2 is expressed during gastrulation in the dorsal ectoderm. Red line indicates the dorsal blastopore lip. (G) At stage 13, Nkx6.2 expression is detected in a broad part of the medial neural plate. At stage 14 (H) and stage 17 (I) Nkx6.2 expression extends through the medial developing hindbrain and spinal neural plate. (J) At stage 34, Nkx6.2 expression is detected in the ventral spinal cord and hindbrain as well as the foregut (red arrowhead). Nkx6.1 and Nkx6.2 expression is confined to the medial part of the neural plate when compared to the pan-neural marker Sox2 expression (compare (C, H) to (K); and (D, I) to, (L)). (N,O) Neither Nkx6.1 (N; red arrowhead) nor Nkx6.2 (O; red arrowhead) expression overlaps with Dbx1 expression (N,O; black arrows) at stage 16. Image published in: Dichmann DS and Harland RM (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx6-2.L nkx6.2 X. laevis Throughout NF stage 10.5 neuroectoderm ectoderm dorsal nkx6-1.L LOC108704117, nkx6.1 X. laevis Throughout NF stage 13 neural plate neuroectoderm nkx6-2.L nkx6.2 X. laevis Throughout NF stage 13 neural plate neuroectoderm nkx6-1.L LOC108704117, nkx6.1 X. laevis Sometime during NF stage 14 to NF stage 17 neural plate neural tube hindbrain spinal cord sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 14 to NF stage 17 neural plate neuroectoderm nkx6-2.L nkx6.2 X. laevis Throughout NF stage 14 to NF stage 17 spinal cord neural plate ectoderm hindbrain neural tube neural groove neuroectoderm dbx1.S dbx, Xdbx X. laevis Throughout NF stage 16 neural plate neuroectoderm mnx1.L hb9, hlxb9, hoxhb9, scra1 X. laevis Throughout NF stage 17 neural plate neural tube neural groove neuroectoderm nkx6-1.L LOC108704117, nkx6.1 X. laevis Throughout NF stage 33 and 34 ventral spinal cord hindbrain foregut neuroectoderm neural tube posterior neural tube anterior neural tube nkx6-2.L nkx6.2 X. laevis Throughout NF stage 33 and 34 ventral spinal cord hindbrain foregut neural tube posterior neural tube anterior neural tube Image source: Published Larger Image Printer Friendly View

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