Xenbase Image ID: 74522

Figure 5. Xenopus akt2 shares a similar expression pattern with appl1 and can rescue the phenotype of appl1 knockdown. A: Whole mount in situ analysis indicates that akt2 is also expressed in pancreas and SD of tadpole-stage embryos (c–e). a, b: Lateral view. c–e: Dissected guts. B: RT-PCR analysis of temporal expression pattern of akt2. ODC was used as the RNA loading control. C: The edema phenotype caused by appl1 Mo1 was rescued by co-injecting 1 ng of akt2 mRNA with 40 ng of Mo1. The co-injection was repeated 3 times with 212 embryos injected in total. ME, moderate edema; N, normal; SE, severe edema. D: Western blot analyses revealed that the down-regulation of akt phosphorylation levels upon appl1 knockdown can be rescued by akt2. In this case, the injection was done at 8-cell stage and all eight blastomeres were injected with reagents indicated. The injected embryos were subjected to Western blot analyses when control siblings reached stage 41. Beta-actin was used as the protein-loading control.Download figure to PowerPoint Image published in: Wen L et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue akt2.L pkbb, pkbbeta, pkbss, prkbb, Protein kinase Akt-2-B, rac-beta, v-akt2 X. laevis Throughout NF stage 35 and 36 brain hindbrain midbrain-hindbrain boundary olfactory region pharyngeal arch eye head region akt2.L pkbb, pkbbeta, pkbss, prkbb, Protein kinase Akt-2-B, rac-beta, v-akt2 X. laevis Sometime during NF stage 41 to NF stage 43 endoderm stomach pancreas alimentary system akt2.L pkbb, pkbbeta, pkbss, prkbb, Protein kinase Akt-2-B, rac-beta, v-akt2 X. laevis Throughout NF stage 7 animal hemisphere Image source: Published Larger Image Printer Friendly View

Return to previous page