Xenbase Image ID: 76175

Fig. 7. Comparison of the spatial expression profile of enpp2a [enpp2.S] and enpp2b [enpp2.L] genes during development. (A) Whole-mount in situ hybridisation with an enpp2a and enpp2b DIG labelled anti-sense RNA probe was performed on embryos from stages 5-41. Animal view at stage 5 (i, ii). Lateral view at stage 21 (iii, iv), stage 26 (v, vi), stage 32 (vii, viii) and stage 37/38 (ix, x). Dorsal is up and anterior is left. The dotted line through the fins (ix) corresponds to plane of section in Ci. (B) Whole-mount in situ hybridisation with an enpp2a and enpp2b DIG labelled antisense RNA probe on stage 32 embryos. Transverse section through the head (i, iv) and trunk (ii, v) levels. Lateral view of the trunk region (iii, vi). Arrowheads indicate the staining in the spinal cord. (C) Whole-mount in situ hybridisation with an enpp2a DIG labelled antisense RNA probe. Transverse section through the fin at stage 37/38 (i). Dorsal view of a stage 41 cleared embryo (ii). ba, branchial arches; df, dorsal fin; dt, distal tubules; e, eye; f, fin; fb, forebrain; fcr, fin crest; fco, fin core; h, heart; lh, lymphatic heart; lv, liver diverticulum; n, notochord; nc, neural crest; np, neural plate; ns, nasal placode; ov, otic vesicle; p, pharynx; pt, pronephric tubules; s, somites; sp, spinal cord; vf, ventral fin. Image published in: Massé K et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 21 somite posterior placodal area neural crest neurogenic placode enpp2.L autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 21 somite neural plate neurogenic placode neural plate border chordal neural plate border optic vesicle enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 26 dorsal fin enpp2.L autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 26 eye pronephric tubule dorsal fin otic vesicle branchial crest pronephric duct anterior ventral blood island notochord enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 32 brain fin otic vesicle forebrain pharyngeal arch branchial arch head mesenchyme floor plate notochord anterior foregut enpp2.L autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 32 brain eye notochord somite pharyngeal arch otic vesicle forebrain pharynx spinal cord hindbrain dorsal fin anterior ventral blood island head mesenchyme enpp2.L autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 37 and 38 otic vesicle fin outer fin head region head liver primordium heart liver diverticulum pronephric tubule posterior cardinal vein enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 37 and 38 eye fin heart head region notochord tail tail fin enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 41 left lymph heart right lymph heart olfactory pit notochord head region fin pharyngeal region enpp2.S autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 5 (16-cell) animal blastomere animal pole enpp2.L autotaxin, enpp2a, enpp2b X. laevis Throughout NF stage 5 (16-cell) animal pole animal blastomere Image source: Published Larger Image Printer Friendly View

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