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Fig. 2. Spatial expression pattern of dhrs3. Whole mount in situ hybridization was employed to examine the spatial expression of dhrs3 at different developmental stages as indicated. (A) Lateral view of a four-cell stage embryo. (B-F Dorsal view of early stage embryos. dhrs3 transcripts were localized in the dorsal blastopore lip and the ridge of neural fold. The expression of rdh10 in stage 11, 13 and 19 are shown in C'D'F' for comparison. (G-I) Frontal view of stage 13, 15, 19 embryos, which showed that dhrs3 expression was absent in the head, but present in the posterior edge of the up-folding neural tube. (J-L) Lateral view of stage 13, 15, 19 embryos, showing dhrs3 expression in ventral bilateral regions (arrow). (M-N Lateral views of tailbud and tadpole stage embryos, showing the expression pattern of dhrs3 and rdh10. The anterior region of a stage 35 embryo showing dhrs3 expression was magnified in (N). dhrs3 expression was detected in the forebrain, midbrain, pharynx, spinal cord and pronephros (arrow). In lateral views (J-N), anterior is to the left. Abbreviations: fb, forebrain; mb, midbrain; p, pharynx; sc, spinal cord; pn, pronephros.
