Xenbase Image ID: 77883

Fig. 6. Osr1/Osr2 promotes lung specification and growth by restricting BMP signaling. (A). Analysis of Osr1/Osr2-MO and control MM-MO stage 35 embryos indicated that expression of bmp4 and BMP-target genes msx1 and hand1 are expanded into the ‘wnt2b-domain’ of the lpm on the Osr1/Osr2-depleted side. Black arrows indicate normal expression, red arrows indicate absent expression and green arrows indicate enhanced expression. (B) Confocal immunostaining of stage 35 embryos for phospho-Smad1/5/8 (green) and fibronectin (red). Scale bars: 50 μm. White arrows in B indicate ectopic pSMAD1 expression in dorsal foregut endoderm. (C) Analysis of nxk2.1, sox2, wnt2b and raldh2 at stage 35 or sftpc at stage 41 in embryos injected with Osr1/Osr2-MOs or MM-MOs and treated at stages 25-35 with either DMSO (vehicle control) or 10 μM LDN193189. Insets show ventral views of wnt2b expression. For analysis of sftpc expression at stage 41, embryos were removed from the LDN193189 at stage 35 and further cultured in DMSO vehicle until stage 41. Black arrows indicate normal expression, red arrows indicate absent expression, green arrows indicate expanded expression and white arrows indicate rescued expression. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wnt2b.L wnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2b X. laevis Throughout NF stage 35 and 36 lateral plate mesoderm lens head brain pharyngeal arch eye otic vesicle pineal gland bmp4.L bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 35 and 36 lateral plate mesoderm heart head brain pharyngeal arch eye msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 35 and 36 lateral plate mesoderm head brain pharyngeal arch eye lens spinal cord epidermis cement gland epibranchial placode hand1.L eHand, hand-1, LOC108712623 X. laevis Throughout NF stage 35 and 36 lateral plate mesoderm heart pharyngeal region head sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 35 and 36 esophagus foregut stomach brain spinal cord sftpc.S SP-C, SPC, surfc, xSP-C X. laevis Throughout NF stage 42 lung Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.6.a Expression Phenotype mislocalised bmp4 expression in lateral plate mesoderm increased amount bmp4 expression in lateral plate mesoderm increased amount hand1 expression in pharyngeal region mislocalised hand1 expression in pharyngeal region mislocalised msx1 expression in lateral plate mesoderm increased amount msx1 expression in lateral plate mesoderm decreased amount wnt2b expression in lateral plate mesoderm Xtr Wt + osr1 MO + osr2 MO + NF35/36 (immunohistochemistry) fig.6.b Anatomical Phenotype abnormal development of foregut abnormal foregut morphology Expression Phenotype increased amount smad1 expression in dorsal foregut Xtr Wt + LDN-193189 + NF35/36 (in situ hybridization) fig.6.c Expression Phenotype increased amount aldh1a2 expression in lateral plate mesoderm mislocalised sox2 expression in foregut increased amount sox2 expression in foregut increased amount wnt2b expression in lateral plate mesoderm Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.6.c Expression Phenotype decreased amount aldh1a2 expression in lateral plate mesoderm decreased amount nkx2-1 expression in lung bud decreased amount sox2 expression in foregut decreased amount wnt2b expression in lateral plate mesoderm Xtr Wt + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.6.c Expression Phenotype decreased amount sftpc expression in lung bud Larger Image Printer Friendly View

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