Xenbase Image ID: 80158

Figure 4. The expression pattern of golph2 during early development. A. RT-PCR analysis of the temporal expression pattern of golph2. UE, unfertilized egg. RT(-), negative control of reverse transcription. Ornithine decarboxylase (ODC) was used as the RNA loading control. B. Western blotting analysis of golph2 expression during Xenopus embryogenesis. Beta-actin served as a protein loading control. C. Whole-mount in situ hybridization was conducted using a digoxigenin-labeled antisense probe for golph2. C. Embryos bisected prior to in situ hybridization. Orientation: animal top, dorsal right (C, D); dorsal top, ventral bottom (E, F), stages as indicated. The expression of golph2 is detected in the dorsal marginal zone and the derivative mesoderm at the gastrula stage. G. Lateral view, anterior towards the left; golph2 expression in the pronephros is detected between stages 25 and 39. Expression of golph2 in the otic vesicle and cement gland is also shown (H). Arrow heads indicate the signal. Image published in: Li L et al. (2012) Li et al. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue golm1.L golph2, gp73 X. laevis Throughout NF stage 10 dorsal marginal zone blastocoel blastocoel roof ventral marginal zone golm1.L golph2, gp73 X. laevis Throughout NF stage 11 dorsal marginal zone mesoderm ventral marginal zone golm1.L golph2, gp73 X. laevis Throughout NF stage 13 mesoderm blastocoel blastocoel roof circumblastoporal collar involuted dorsal mesoderm involuted ventral mesoderm golm1.L golph2, gp73 X. laevis Throughout NF stage 17 axial mesoderm circumblastoporal collar mesoderm archenteron gastrocoel roof plate golm1.L golph2, gp73 X. laevis Throughout NF stage 25 pronephric kidney otic vesicle cement gland proctodeum pronephric mesenchyme golm1.L golph2, gp73 X. laevis Throughout NF stage 31 pronephric kidney otic vesicle cement gland pronephric tubule golm1.L golph2, gp73 X. laevis Throughout NF stage 35 and 36 pronephric kidney otic vesicle cement gland pronephric tubule golm1.L golph2, gp73 X. laevis Throughout NF stage 39 pronephric kidney pronephric tubule cement gland pronephric duct late proximal tubule early proximal tubule early distal tubule late distal tubule head region mouth primordium Image source: Published Larger Image Printer Friendly View

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