XB-IMG-81524
Xenbase Image ID: 81524
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Fig. 4. Spatial expression of pax1 transcripts during Xenopus development. Whole-mount in situ hybridization was carried out using a digoxigenin-labeled riboprobe. A: Lateral view, ante- rior is to the right. A: At stage 22, pax1 is expressed in the branchial arches and weakly in the somite (red arrows). B: The pax1 expression is increased and its localization advances pro- gressively to caudal region of the somites in the stage 24 (B), stage 26 (C), and stage 35 embryos (D). E: Magnification of the dorsal region of D at the somite level. Black arrows indicate the intersomitic space. F: Whole-mount in situ hybridization with pax1 sense probe was used as negative control. G,H: Transverse sections of stage 35 embryos at the branchial arches level (G) and trunk (H). Nieuwenkoop-Faber stages are indicated on the lower left corner. BA, branchial arches; N, notochord; NT, neural tube; PE, pharyngeal endoderm; S, somite. Image published in: Sánchez RS and Sánchez SS (2013) Copyright © 2013. Image reproduced with permission of the Publisher.
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