Xenbase Image ID: 82462

Fig. 4. Spatial expression profiles of polypyrimidine tract binding protein (PTB) genes in late tailbud embryos. Whole-mount in situ hybridization followed by longitudinal and transverse sections were performed to examine the spatial expression of ptbp1, ptbp2 and ptbp3 in late tailbud embryos. (A) ptbp1 (a) lateral view of a stage 34 embryo, the white lines indicate the level of transverse and longitudinal sections shown in panels b-i, (b-d) longitudinal sections of the head along the dorso to ventral axis, (c longitudinal section of the trunk realized at the same level as panel c, (e-i) transverse sections along the anterior to posterior axis, (i higher magnification view of panel i. Black arrowheads point to the CMZ of the retina (B) ptbp2 (a) lateral view of a stage 34 embryo, the white lines indicate the level of transverse and longitudinal sections shown in panels b-g, (b-d) longitudinal sections of the head at the level of the brain, (d longitudinal section of the trunk realized in the same plan as panel d, (e-g) transverse sections of head and trunk. Black arrowheads point to the neuronal retina. (C) ptbp3 (a) lateral view of a stage 34 embryo, the white lines indicate the level of transverse and longitudinal sections shown in panels b-j, (b-d) longitudinal sections of the head along the dorso to ventral axis, (c longitudinal section of the trunk realized at the same level as panel c, (e,f) transverse sections of the head at the level of the diencephal. (g,h) higher magnification views of transverse sections of the head at the level of the rhombocephal, (i,j) transverse sections of the trunk, (j higher magnification view of panel j. Black arrowheads and arrows point respectively to the posterior lens and the hypothalamic labeling. Abbreviations used are: da, dorsal aorta; di, diencephal; dm, dermatome; h, heart; im, intermediate mesoderm; m, myotome, nc, notochord, ov, otic vesicle; rb, rhombocephal; sc, spinal cord; va, ventral aorta. Image published in: Noiret M et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue ptbp1.S hnrnp I, hnrnp-I, hnrpi, ptb, ptbp1-a, ptbp1-b, vgrbp60 X. laevis Throughout NF stage 33 and 34 retina intermediate mesoderm eye ciliary marginal zone otic vesicle dermatome pharyngeal arch mandibular arch hyoid arch branchial arch head mesoderm endoderm ptbp2.L LOC108714373, LOC108715653 X. laevis Throughout NF stage 33 and 34 retina intermediate mesoderm eye head region central nervous system brain spinal cord hindbrain diencephalon ptbp3.S rod1 X. laevis Throughout NF stage 33 and 34 intermediate mesoderm notochord eye dermatome hypothalamus lens otic vesicle head region pharyngeal arch mandibular arch hyoid arch branchial arch dorsal aorta blood vessel Image source: Published Larger Image Printer Friendly View

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