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Figure S5. Expression of xRARα, xRARβ, and xRARγ in Xenopus embryos
Whole mounts are oriented with anterior toward the right. Expression of RARα and RARγ is detectable from the onset of gastrulation (stage 10) while the first signal for RARβ is detected at the early tailbud stage (stage 25). At mid-gastrula stage (stage 11), RARα is expressed as a narrow ring around the blastopore. As gastrulation proceeds, expression intensifies and the signal around the blastopore widens preferentially on the dorsal side except in the midline, which exhibits a low level of transcripts. During neurulation (from stage 14), transcripts are found predominantly in the neurectoderm, evenly distributed along the anterior-posterior axis, with the exception of a region at the anterior end for which transcripts are largely reduced. At the tailbud stage (stage 30), RARα is predominantly expressed in the spinal cord and the posterior hindbrain, in the eye and the posterior branchial arches. During gastrulation (stage 10), expression of RARγ is more widespread than RARα expression. Transcripts are present in the mesodermal marginal zone as well as in the ectoderm. By the neurula stage (from stage 14), the staining separates into anterior and posterior domains, thus creating a gap with no RARγ transcripts. Expression remains localized to the posterior and anterior ends of the embryo at tailbud stages (stage 30) and is mainly restricted to the branchial arches and the tip of the tailbud. RARβ transcripts are detected at much lower level than RARα and RARγ at the examined stages. The signal is restricted to the caudal part of the hindbrain and the anterior spinal cord. At the late tailbud stage (stage 32), RARβ is strongly expressed in the most posterior branchial arches. d, dorsal views; l, lateral views; f, frontal views.
doi:10.1371/journal.pgen.0020102.sg005
