XB-CLONE-12442128
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Clone Name: gli3-AP451
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number NP-451.
Xenopus laevis GLI family zinc finger 3.
4747bp (ORF).
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: pCS2+MT XGLi3_FullLength
[+]
tTGTtnnTTctTctcgCTCCTAnGTTaTTGCaTAnTaGCAnGaAACTTGCTTTCTTCTGcnccnnnnnnnngnnAGAACTCATGTCCCCAATAGCCATGT
TAGAtgtactAACAGGTATGGCTGGGAATGTCAGAGATGCTCGAGGAGTGGTAAGGCGTGAAGAATTACGGGAGAGATTCTGGATGATACTTGGACTTAG
AACTCCAGAAATGAGGCTGGCATGATCACCATCATCAATGATAGCATCAAAATCAATTTGTACCCCATCCAAACTGTTATCAATGTTATCGACTGTGCTA
GTCACTTGGTTAGCCCCAGGTGAAAGAAGCTTGGATGAGTTTGTATTGTTACTTTCTTGTAAAAGACAGTTTGCTTCCATACCGGGTTCAGAGCCTGATT
TCATAATTTGTTGAGGGTATGTTGCTGTTTGGTCATACAACTGACCGGAGAAGTTTTGTGCGTTTGCACAGTGCTGTTGTGGGTGACTCTGCATTTCCAT
CTTAATACTGTTCACTTTGAAAATGTGTTGGTTAGTGTCATATATTTGCTCTTGCAGATATGGCAGGTTGTTTCTGTGTAAATTCTGCCTGCTATTTCCA
CTAGAGTTTACAAAAGACTggAAATTCTTTGCAAGGGTtggCTGCTGCTCGCCACCattTtGAGTagtTGACAggaAACTTcgaGTTCCGTCAgntGtgt
TTCCATTTTTGCCCCTGAtggtgTctgATtagAtcgAGTCaCCCCCCAgncaAatgnat
Construction:
Insert:
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| Size: 4747 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pCS2+
Type: phagemid
5' Restriction Site: XbaI
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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SP6 |
NotI |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T3 |
BamHI |
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Suppliers:
Search for clone at:
EXRC
