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Summary Attributions
XB-CLONE-1855632

Clone Name: XL215a01 Gene: cdc27.S Species: Xenopus laevis  
 

Sequence:

5' EST: BJ626470 [+]

3' EST: BJ642857 [+]

Library:

Name: NIBB Mochii normalized Xenopus early gastrula libraryExternal Dbs: Unigene laevis cDNA library

Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).

Anatomy: whole organismStage: NF stage 10.5
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pBluescript SK-

Type: phagemid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems