Summary Attributions

Clone Name: IMAGE:6878724	Gene: tubb4b.L	Species: Xenopus laevis

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Sequence:

5' EST: CB180500 [+]

gggaggcgtttcaaggcaacaggagagatcagtaggacagagcagtttactacagacaccatgagggaaatcgtgcacttgcaggctggacagtgcggca
atcagattggtgccaagttctgggaagtgatcagcgatgagcatggaattgacccaactggtgcctaccatggggacagtgacctgcagctggagagaat
caatgtgtattacaatgaagcaacaggtggcaagtacgttccccgcgctgtgctggttgatcttgaacctgggaccatggactctgtgcgctcagggcca
tttgggcagatattcaggcctgacaactttgtatttggtcaaagtggtgctggaaacaactgggccaagggccactacacagagggagcagaactggttg
actccgtgttggatgttgtaaggaaagaagcagaaagctgtgactgtctccagggcttccagctgactcactctctggggggtggcactggctctggcat
gggtacactgctcatcagcaaaatcagggaagagtacccagacagaatcatgaacacgtttagtgtagtgccctcccctaaagtgtctgacacagtagta
gagccatacaacgccaccctgtcagtgcaccagcttgtagagaaaactgcatgagacctactgtatagaatatgaagccctgtaatgaaatcttgctttc
cgaacccctaaaactcacacaccccacctatgggggacctgaaaccacttggg

3' EST: CB560206 [+]

aacactggtaacattgaaactggagagaagnaatgcaggtgacaataaatggaacaaacgagcagaaatggatgaataatttacaagtgataaggagctt
taggcattttcctcctcttccccttcctcaaactctccctcctcctcagccgtggcatcctggtactgttggtactcagacaccaggtcgttcatgttgc
tctcggcctcagtgaattccatctcatccatgccctcacctgtgtaccagtgcaagaaggcctttctgcggaacatggcggtgaactgctcagagatgcg
cttgaacagctcctggatggctgtgctgttgccgatgaaggtggcagacattttcaggcctctgggtgggatgtcgcagactgcggtcttaacgttgttg
gggatccattccacaaagtagctgctgttcttgttctgcacattcagcatctgctcatccacttctttcatggacatgcggcccctgaaaatggcagcta
cagtcagatagcggccatgacgggggtcacaggcagccatcatgttcttggcatcgaacatctgttgggtgagttctggcacggttaaggcacggtactg
ctggctacccctgctggtgagtggagcaaagccaggcatgaagaagtgcagacgggggaatgggaccatattgacagccagtttgcgcaggtcagcattg
agctggccagggaatcgcaggcaggtagttaccccactcattgtggctgagaccaagtggttcaagtccccatangtgggttgtgggtaaatttttaggg
gttcctgaagcagatatcctacaaggctttcattatctataacagtaggtctcatcagtgtttctctacaa

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Library:

Name: NICHD_XGC_Tad1

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 54 to prometamorphosis stage
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pDNR-LIB Type: plasmid 5' Restriction Site: SfiI 3' Restriction Site: SfiI

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Usage:

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Suppliers:

Search for clone at: Open Biosystems