Summary Attributions

Clone Name: mef2a-861	Gene: mef2a.L	Species: Xenopus laevis
Description: EXRC Clone Number 861. Xenopus laevis myocyte enhancer factor 2A. Image EST 5536990, Accession BC046368. Full length. To generate probe for in situ.

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Sequence:

Full length sequence: BC046368 [+]

GGCAACAGTTGGACCTTGCTGCTGGCCAGAGTCCCTTCAGTTACTATACATCTTCCAGTAGACCCTTTGTTTAAAACGTGGCATGAAGTTGACTAATACG
TTTTCTCTAAAAGAATAAAGCCTTGAGCTGCACAAATAAGAAGTTCAAACACGGCTGGCCATGCATTAATTTTTTGGATCAAGCTATTTGTGTGGACTGT
AAAGTTGCTGTAGCTGGCAAAGGGAGAGAACAACGCAACCATGGGAAGGAAGAAAATACAAATTACGCGGATAATGGACGAACGAAACAGACAGGTAACA
TTTACCAAGAGAAAGTTTGGACTGATGAAGAAGGCTTACGAACTGAGCGTACTCTGTGACTGTGAAATTGCACTGATTATTTTCAATAGCTCTAACAAGC
TGTTTCAATATGCCAGTACGGACATGGACAAAGTCCTTCTCAAGTACACCGAATACAATGAGCCCCATGAGAGCAGAACCAACTCTGACATTGTTGAGAC
TCTCAGAAAGAAAGGCTTAAATGGTTGTGAAAGCCCAGATGATCAGAGATACTTTGAGGAGGAGACATTCAGCAAACTAATTGAAGATAGTGATTTTGTT
TTCAAGCGAGACCCTGCACTGAACAAGAAGGAAAACAGAGGTTGTGACAGCCCGGACCCTGATGGCTCCTACGTACTCACCCCCCACACCGAGGAAAAGT
ACAAAAAAATTAACGAGGAATTTGATAATATGATGCGCAGTCATAAAATCTCACCTGGATTACCACAGCAAACCTTTCCAATGTCTGTCACTGTACCGGT
GTCCAACCCAAACACATTAACGTACAGTTCTCCAGGGAACACAATGGTTACTGCTTCATTAGCTGCCAGTGCATCATTAACAGATGCAAGAATGCTGTCC
CCACCTCCAACGACACTGCATCGAAATGTGGTTTCCCCAGGGCTACCCCAGCGGCCTCCTAGCACGGGAAATGCTGGTGTAATGTTGTGTTCGTCTGATC
TCTCCGTGCCAAATGGAGCAGGTACCAGTCCAGTAGGTAATGGGTTTGTAAACCCTCGCGCTTCTCCAAGCCACTTAGGACCAACGGGAGGGAATGTGCT
GGGGAAAGTGATGCCTACAAAATCTCCCCCACCCCCTGGAGGGAACCTTGTTATGAACAGCCGAAAACCTGACCTACGTGTGGTTATTCCTCCTTCCAGT
AAAGGCATGATGCCTCCTTTGAACACCCAAAGAGTAACCAGTTCACAAGGTACTCAGCCACTTGCCACCCCAATTGTTTCAGTAGCTACTCCCAGTTTAG
CCCCTCAGGGGCTGATTTATTCTGCAATGCCTACTGCCTACAATACAGACTATCCTTTGACCAGTGCAGATCTGTCAATGCTGCAGGGGTTTAACTCCCC
AGGAATATTGCCTCTAGGACAGGTTTCAGCCTGGCAACAGCATCACGTAGGACAAGCAGCCCTCAGCTCCTTTGTGGCTACTGGACAATTATCTCAGGGT
TCTAATTTGTCCATCAACACAAACCAGAACATCAATATAAAGTCCGAGCCCATATCACCCCCCCGTGATAGGATCACTCCTTCTGGATTTCAATCCCATC
AGCACCACCAACACCAACCTCGCCCAGAAATGGACAGCCTGAGCAGCTCCAGCAGTTCCTATGATGGCAGCGACCGAGAGGACGTCAGAAATGACTTTCA
TTCACCGATTGGCTTGGGAAGACCTGCCAATAACGAGGACAGGGACAGCCCCTCTGTGAAACGCATGAGGATGGATGCTTGGGTGACATAAATATTTCTC
CAGACACCTTTTATGGCGAGGAACCCTTGAGCATATCTAAAACTATATGTCACAGGTGCACAAGTTGTATATCCGCATTTGTGTCATACACACATCCATC
AGGTACCTGCTGCAATGACTGGAAACACCTACAACTTTCTGCAGTGATGTGGCACTTAATGTGGACTTTCTAACAACTCCGGGGGAAGCAATGCATTAGG
CCTCAGTGTAATCATACTTTTCTGAAACTACAATAGTAAATGACCCTTTTAAAAGGGAAATGTGGAGGGTTGAAACCTTTCCGCGCTTGGGGAGAGGCTC
CTATCCCCCCCATCCTTATCCCTGTGCTTTTAACCCCTTTGCTGACAAAAGGGCATTCAGTGCATTGTATTAGCCCATCTGGAAGCACATGGGTTTGACA
CTTGGCTTGATAGAACAAACAGGACAATGAATGCAGGAAACAAAGAAAGCAATACTGTAAATGTAAAACAATAATAAATGTTTTATCCAAAATTAATACC
TACAGATCCCTCTTTGAAAGGGAGCAGGTGAAGGGCTGAAGCCACGAGAACTATTTTTGCACGTGCGACACAAACAGCAATGATATCAGGTCAGAAAGTT
TTATGTTTCGAATCTAGGGAGTCATAGGGCATTGTATTGGGGGAGGTTAATAAATAAATAAAAAAAAAAAAAAA

5' EST: BQ731273 [+]

caacagttggaccttgctgctggccagagtcccttcagttactatacatcttccagtagaccctttgtttaaaacgtggcatgaagttgactaatacgtt
ttctctaaaagaataaagccttgagctgcacaaataagaagttcaaacacggctggccatgcattaattttttggatcaagctatttgtgtggactgtaa
agttgctgtagctggcaaagggagagaacaacgcaaccatgggaaggaagaaaatacaaattacgcggataatggacgaacgaaacagacaggtaacatt
taccaagagaaagtttggactgatgaagaaggcttacgaactgagcgtactctgtgactgtgaaattgcactgattattttcaatagctctaacaagctg
tttcaatatgccagtacggacatggacaaagtccttctcaagtacaccgaatacaatgagccccatgagagcagaaccaactctgacattgttgagactc
tcagaaagaaaggcttaaatggttgtgaaagcccagatgatcagagatactttgaggaggagacattccgcaaactaattgaagatagtgattttgtttt
caagcgagaccctgcactggaacaagaaaggaaaacagaggttggtgacagcccgggaccctgatgggctcccaacgtactccacccccccccaccgagg
gaaaaagtacaaaaaaatttacccgaggaaattggataataatgagtgcgccgcgcataaaaatcctccccctggaattacccacagcaaaaccctttcc
aatgggccggccactggtaaccgggggtcccaacccccaaacacctttaaagggtacacgtgttctcccgggggaaaacaaaggggctcacggcgctcat
ttaacctggccaagggccttcacattaacgc

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Library:

Name: NICHD_XGC_Emb4

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 31 to NF stage 32
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: NotI 3' Restriction Site: SalI

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Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 SalI EcpRI can also be used.

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Suppliers:

Search for clone at: EXRC