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Summary Attributions
XB-CLONE-2493607

Clone Name: IMAGE:8883277 Gene: nedd8 Species: Xenopus tropicalis  
 

Sequence:

5' EST: EL838713 [+]

3' EST: EL838714 [+]

Library:

Name: NICHD_XGC_trop_25External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.

Anatomy: whole organismStage: NF stage 25
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems