XB-CLONE-3055305
Clone Name: IMAGE:7848537
|
Gene:
hmg20a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT533667
[+]
atggggacaaagtgaagacttttattaagaaatatgacatgcagttctgcaaacaatacagtctagagaagtttgcaaagatgatgtccagtgtattctt
ttctgcagtgtctttggcaaagagaaccatgtgagcgataaaaaaaagttgaagttccaaatgagttaaacacatgaaacatgttctcttcacccttcca
gctggcttagaacctctctgacacccgagataataacctcactctccttagggtgtgtaagcacagcattctgcaaccggctcatgtataagtcaatggt
ctctaaggttggaatttctgtggtacctggaattggcacagactgcagacagtgagtcagtgcctggcggacgctctgtaaatgctgctgtaacagagag
tttctttcctgttcttggttcagctcagcttccagtctctgaacagctgaacgcatgctttccacatgcttctgtagggctgcattacgttcctcaaact
ccatattggacttccgtagttgcctcagctctgcctcccgtgctttactgtgattcagaaactcctctgtaaagatagggatgtcaaagattggcctttc
cttcagaatggattctttctctgtgttgattgggactccacgtactccctcttgcctttgctgccttcccttctgacgactctgggccttgcggctgtat
gtttggtaggcatctgtgttctggtactgctgcaattcctttgtgtagcgctctttatctttttctgcttcatctaagtaatgctgtttctcatgggcag
gtagtttgctccactcactacctacaatcctggtgatctctgggaatggaacatctggacgctctgtcctgagctgctcccttctttca
3' EST: DT533666
[+]tagggcgagagggctgtccggggctactgggctggtttagggaaatcgggactgttgggcagtatgaaagctgtgtagggaaaaccatgcaggcacccta
gcaagcgctctcccgatgtctgcagctcactgacagcaaggtggcccgataacccaaggcagattgtcttgctgtgtgacaacagactgaggactttaac
actttccaagaagacctccagcctgggctgcagccttgagcaaatagacaaatggaaaatacagcctctgcagcacccccttccagtgaggaccttgttg
ttgacacaaaagaaaatgctgagccaccattttgtgggatctctctctcaggaagctcccaggcaccttttcatccacaatcccctacccttcagcaaga
tgaaagggatgagctgattttgcagcaaagtggggagcagcaactgggaaacagtggagaattaaggcaggaggaagagttgccaaagacacggcggggt
ggctggactaaaggacggaagcgaaaaagaagcccccgtgacaacaatgcaccaaaagcaccgttaacaggatatgtgcgctttatgaatgaaagaaggg
agcagctcaggacagagcgtccagatgttccattcccagagatcaccaggattgtaggtagtgagtggagcaaactacctgcccatgagaaacagcatta
cttagatgaagcagaaaaagataaagagcgctacacaaaggaattgcagcagtaccagaacacagatgcctaccaaacatacagccgcaaggcccagagt
cgtcagaagggaaggcagcaaaggcaagagggagtacgtggagtcccaaatcacacagagaaagaatccattct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: