XB-CLONE-3063548
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Clone Name: IMAGE:7845444
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Gene:
ddx20
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528222 
[+]
tccagaggactgggaaacctatatgcatcgaattggaagagctggacggtttggtactcatggcctctctgtaactttctgttgccatggggaagaggag
aatatgatgatgggaattgcaatgaaatgcaatcttaagcttttgcctttaccagatccaataccagatggtttaatggaagagacctgtaactgggata
ttgaagttactacggtgaagaccataccttcctcttctgagaatcaaggggtttgtacaacagaagtacttagcagcaacccactgccagaaacttacca
aaataaaaaggccaccaaaacacattctaagacaaaaaagacctcaatgaaactttatcctgagaaggaaagtaagaagggggagaccactgtaaattgt
gatggaatccttaaaccacctaaagatgtggccaagccaaagaagccagtggataacaccgtaaacaacagtgcaaatatggcagagttgcaaaactcgc
tacctaaaataccaagcttggcatccttcaagcgctcagttgcttgtacatcatcatttgcagagctagttgaagactatgaacaatttatcaaagaagg
tttggaaaaacatgtggaaatagtgcgtgtgtatactggccctggtgacaaaaatgagctgaaatctgaaaatctagaacaggaaagaactgatgatggg
ggcacttcagaagcaatgagtactcctgaaaagtgtggatttgctgagagttctgttagtgaaagtgatagaagtgcatcctgctcttcttcagtcagtg
aagaaagcaatgaagactctgatattccag
3' EST: DT528221 [+]gaatcacttattcctttaataagcattacactgttgaaaaatgagtaggtcaatagtcagctaagactaaaccctccaaatacagttgctgactgaaaca
gtgaaatacttgaaggtagagaaggtatataagtaatcatgcagaatttacaacataatgaaaaaaagaaatatatagcattttcaagctggtttactat
tgtgtattaaaccaattttatgtatttgttggagttttgcaatttctgttttaaaccagaaaaatgtgatcaccagtgaatggtgaaggttgtttaaggc
atagaagaatattaaaatgtaaaccataaaccactgccttattattaacataatttgagtttgtcatggctttagcatttcatggaggtacacagattgc
atgtgatatgcagacatccagcttgaacgcttgtacagattacggtagtaagaatttgatacagtagaatggtatgtttgccatgcttgataataggacc
tccagtagtcagcatagctcgtggtgccatatggaaatctactagggtttctatattccaactggtacaaatcatcttgtctttccgaacagagttcacg
tctagacacaggtgttgtgtacttactccgtcttctagatttggtattgctcacaaatggcatttcacctttatatttatgagctttcatcacatttcct
tcttttgtcaagtcagaaatattatctgtgttgagttcacaaacagtaggttcaattgtcctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
